FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068050924> ?p ?o ?g. }

• W2068050924 endingPage "11836" @default.
• W2068050924 startingPage "11828" @default.
• W2068050924 abstract "We have measured the kinetics of inorganic phosphate (Pi) release during a single turnover of actomyosin nucleoside triphosphate (NTP) hydrolysis using a double-mixing stopped-flow spectrofluorometer, at very low ionic strength to increase the affinity of myosin-ATP and myosin-ADP-Pi to actin. Myosin subfragment 1 and a series of nucleoside triphosphates were mixed and incubated for approximately 1-10 s to allow NTP to bind to myosin and generate a steady state mixture of myosin-NTP and myosin-NDP-Pi. The steady state intermediates were then mixed with actin. The kinetics of Pi release were measured using a fluorescent probe for Pi, based on a phosphate binding protein [Brune et al. (1994) Biochemistry 33, 8262-8271]. These data are correlated with quenched-flow data, where the extent of the rapid burst of hydrolysis during the first turnover of ATP hydrolysis was followed by chemical quenching of the reaction mix at various times after rapidly mixing ATP and myosin subfragment 1. From the double-mixing actomyosin measurements, the kinetics of Pi release are biphasic. The fast phase corresponds to Pi release from the associated actomyosin-ADP-Pi complex. The slow phase measures the rate of the cleavage step on associated actomyosin. At saturating actin, there is a correlation between the amplitude of the fast phase and the size of the Pi burst observed by quenched flow in the absence of actin: the size of this phase corresponds to the amount of myosin-ADP-Pi formed during the first mix. For ATP at 20 degrees C the rate of the Pi release step is 75 (+/-5) s-1, 25-fold larger than the cleavage step, which is the rate-limiting step of actomyosin ATP hydrolysis at saturating actin. The rate constant of Pi release varies only slightly with nucleoside structure. The rate constant of the slow phase of the Pi release (measuring cleavage) is highly dependent upon the structure of the NTP substrate." @default.
• W2068050924 created "2016-06-24" @default.
• W2068050924 creator A5029073594 @default.
• W2068050924 creator A5030461994 @default.
• W2068050924 creator A5078281981 @default.
• W2068050924 date "1997-09-01" @default.
• W2068050924 modified "2023-09-24" @default.
• W2068050924 title "Kinetics of Nucleoside Triphosphate Cleavage and Phosphate Release Steps by Associated Rabbit Skeletal Actomyosin, Measured Using a Novel Fluorescent Probe for Phosphate" @default.
• W2068050924 cites W124285527 @default.
• W2068050924 cites W1535644751 @default.
• W2068050924 cites W1582432526 @default.
• W2068050924 cites W1678204546 @default.
• W2068050924 cites W1964389615 @default.
• W2068050924 cites W2010675441 @default.
• W2068050924 cites W2044597760 @default.
• W2068050924 cites W2066853635 @default.
• W2068050924 cites W2077074448 @default.
• W2068050924 cites W2078635406 @default.
• W2068050924 cites W2081069785 @default.
• W2068050924 cites W2114424130 @default.
• W2068050924 cites W2124754866 @default.
• W2068050924 doi "https://doi.org/10.1021/bi970540h" @default.
• W2068050924 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9305974" @default.
• W2068050924 hasPublicationYear "1997" @default.
• W2068050924 type Work @default.
• W2068050924 sameAs 2068050924 @default.
• W2068050924 citedByCount "157" @default.
• W2068050924 countsByYear W20680509242012 @default.
• W2068050924 countsByYear W20680509242013 @default.
• W2068050924 countsByYear W20680509242014 @default.
• W2068050924 countsByYear W20680509242015 @default.
• W2068050924 countsByYear W20680509242016 @default.
• W2068050924 countsByYear W20680509242017 @default.
• W2068050924 countsByYear W20680509242018 @default.
• W2068050924 countsByYear W20680509242019 @default.
• W2068050924 countsByYear W20680509242020 @default.
• W2068050924 countsByYear W20680509242021 @default.
• W2068050924 countsByYear W20680509242022 @default.
• W2068050924 countsByYear W20680509242023 @default.
• W2068050924 crossrefType "journal-article" @default.
• W2068050924 hasAuthorship W2068050924A5029073594 @default.
• W2068050924 hasAuthorship W2068050924A5030461994 @default.
• W2068050924 hasAuthorship W2068050924A5078281981 @default.
• W2068050924 hasConcept C104317684 @default.
• W2068050924 hasConcept C121332964 @default.
• W2068050924 hasConcept C12554922 @default.
• W2068050924 hasConcept C125705527 @default.
• W2068050924 hasConcept C141315368 @default.
• W2068050924 hasConcept C148898269 @default.
• W2068050924 hasConcept C181199279 @default.
• W2068050924 hasConcept C185592680 @default.
• W2068050924 hasConcept C23265538 @default.
• W2068050924 hasConcept C2776352584 @default.
• W2068050924 hasConcept C2776543447 @default.
• W2068050924 hasConcept C2777132085 @default.
• W2068050924 hasConcept C2779564974 @default.
• W2068050924 hasConcept C512185932 @default.
• W2068050924 hasConcept C53009064 @default.
• W2068050924 hasConcept C55493867 @default.
• W2068050924 hasConcept C62520636 @default.
• W2068050924 hasConcept C6997183 @default.
• W2068050924 hasConcept C86803240 @default.
• W2068050924 hasConcept C94412978 @default.
• W2068050924 hasConceptScore W2068050924C104317684 @default.
• W2068050924 hasConceptScore W2068050924C121332964 @default.
• W2068050924 hasConceptScore W2068050924C12554922 @default.
• W2068050924 hasConceptScore W2068050924C125705527 @default.
• W2068050924 hasConceptScore W2068050924C141315368 @default.
• W2068050924 hasConceptScore W2068050924C148898269 @default.
• W2068050924 hasConceptScore W2068050924C181199279 @default.
• W2068050924 hasConceptScore W2068050924C185592680 @default.
• W2068050924 hasConceptScore W2068050924C23265538 @default.
• W2068050924 hasConceptScore W2068050924C2776352584 @default.
• W2068050924 hasConceptScore W2068050924C2776543447 @default.
• W2068050924 hasConceptScore W2068050924C2777132085 @default.
• W2068050924 hasConceptScore W2068050924C2779564974 @default.
• W2068050924 hasConceptScore W2068050924C512185932 @default.
• W2068050924 hasConceptScore W2068050924C53009064 @default.
• W2068050924 hasConceptScore W2068050924C55493867 @default.
• W2068050924 hasConceptScore W2068050924C62520636 @default.
• W2068050924 hasConceptScore W2068050924C6997183 @default.
• W2068050924 hasConceptScore W2068050924C86803240 @default.
• W2068050924 hasConceptScore W2068050924C94412978 @default.
• W2068050924 hasIssue "39" @default.
• W2068050924 hasLocation W20680509241 @default.
• W2068050924 hasLocation W20680509242 @default.
• W2068050924 hasOpenAccess W2068050924 @default.
• W2068050924 hasPrimaryLocation W20680509241 @default.
• W2068050924 hasRelatedWork W1594210443 @default.
• W2068050924 hasRelatedWork W1969761928 @default.
• W2068050924 hasRelatedWork W1995294318 @default.
• W2068050924 hasRelatedWork W1995586883 @default.
• W2068050924 hasRelatedWork W2018365501 @default.
• W2068050924 hasRelatedWork W2068050924 @default.
• W2068050924 hasRelatedWork W2164668862 @default.
• W2068050924 hasRelatedWork W2318597317 @default.
• W2068050924 hasRelatedWork W3122650266 @default.
• W2068050924 hasRelatedWork W342450364 @default.

• next