FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068087647> ?p ?o ?g. }

• W2068087647 endingPage "e27" @default.
• W2068087647 startingPage "e27" @default.
• W2068087647 abstract "In the present research, a rapid, simple and efficient chemoselective method for the site-directed incorporation of tailor-made polymers into protein to create biocatalysts with excellent properties for pharmaceutical industrial purpose has been performed. First we focused on the protein engineering of the Geobacillus thermocatenulatus lipase 2 (BTL2) to replace the two cysteines (Cys65, Cys296) in the wild type enzyme (BTL-WT) by two serines. Then, by similar mode, a unique cysteine was introduced in the lid area of the protein. For the site-directed polymer incorporation, a set of different tailor-made thiol-ionic-polymers were synthesized and the protein cysteine was previously activated with 2,2-dithiodipyridine (2-PDS) to allow the disulfide exchange. The protected BTL variants were specifically modified with the different polymers in excellent yields, creating a small library of new biocatalysts. Different and important changes in the catalytic properties, possible caused by structural changes in the lid region, were observed. The different modified biocatalysts were tested in the synthesis of intermediates of antiviral and antitumor drugs, like nucleoside analogues and derivatives of phenylglutaric acid. In the hydrolysis of per-acetylated thymidine, the best biocatalyst was the BTL*-193-DextCOOH , where the activity was increased in 3-fold and the regioselectivity was improved, reaching a yield of 92% of 3’-O-acetyl-thymidine. In the case of the asymmetric hydrolysis of dimethyl phenylglutarate, the best result was found with BTL*-193-DextNH2-6000, where the enzyme activity was increased more than 5-fold and the enantiomeric excess was >99%." @default.
• W2068087647 created "2016-06-24" @default.
• W2068087647 creator A5020692034 @default.
• W2068087647 creator A5028391028 @default.
• W2068087647 creator A5061655735 @default.
• W2068087647 creator A5077558844 @default.
• W2068087647 date "2013-02-12" @default.
• W2068087647 modified "2023-10-08" @default.
• W2068087647 title "Novel enzyme-polymer conjugates for biotechnological applications" @default.
• W2068087647 cites W1963683058 @default.
• W2068087647 cites W1964048230 @default.
• W2068087647 cites W1965035647 @default.
• W2068087647 cites W1966036307 @default.
• W2068087647 cites W1966793537 @default.
• W2068087647 cites W1989139032 @default.
• W2068087647 cites W2011200949 @default.
• W2068087647 cites W2025634036 @default.
• W2068087647 cites W2029582401 @default.
• W2068087647 cites W2038303118 @default.
• W2068087647 cites W2052466890 @default.
• W2068087647 cites W2061208926 @default.
• W2068087647 cites W2068447470 @default.
• W2068087647 cites W2103454955 @default.
• W2068087647 cites W2124544537 @default.
• W2068087647 cites W2131448603 @default.
• W2068087647 cites W2133436228 @default.
• W2068087647 cites W2145786098 @default.
• W2068087647 cites W2165489120 @default.
• W2068087647 cites W2531769352 @default.
• W2068087647 cites W4236440733 @default.
• W2068087647 cites W4300859091 @default.
• W2068087647 doi "https://doi.org/10.7717/peerj.27" @default.
• W2068087647 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3628993" @default.
• W2068087647 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23638362" @default.
• W2068087647 hasPublicationYear "2013" @default.
• W2068087647 type Work @default.
• W2068087647 sameAs 2068087647 @default.
• W2068087647 citedByCount "14" @default.
• W2068087647 countsByYear W20680876472015 @default.
• W2068087647 countsByYear W20680876472016 @default.
• W2068087647 countsByYear W20680876472017 @default.
• W2068087647 countsByYear W20680876472018 @default.
• W2068087647 countsByYear W20680876472019 @default.
• W2068087647 countsByYear W20680876472021 @default.
• W2068087647 countsByYear W20680876472022 @default.
• W2068087647 countsByYear W20680876472023 @default.
• W2068087647 crossrefType "journal-article" @default.
• W2068087647 hasAuthorship W2068087647A5020692034 @default.
• W2068087647 hasAuthorship W2068087647A5028391028 @default.
• W2068087647 hasAuthorship W2068087647A5061655735 @default.
• W2068087647 hasAuthorship W2068087647A5077558844 @default.
• W2068087647 hasBestOaLocation W20680876471 @default.
• W2068087647 hasConcept C104317684 @default.
• W2068087647 hasConcept C143065580 @default.
• W2068087647 hasConcept C147816474 @default.
• W2068087647 hasConcept C154881586 @default.
• W2068087647 hasConcept C161790260 @default.
• W2068087647 hasConcept C178790620 @default.
• W2068087647 hasConcept C181199279 @default.
• W2068087647 hasConcept C185592680 @default.
• W2068087647 hasConcept C191503008 @default.
• W2068087647 hasConcept C21951064 @default.
• W2068087647 hasConcept C2779201268 @default.
• W2068087647 hasConcept C521977710 @default.
• W2068087647 hasConcept C55493867 @default.
• W2068087647 hasConcept C9418097 @default.
• W2068087647 hasConcept C94412978 @default.
• W2068087647 hasConceptScore W2068087647C104317684 @default.
• W2068087647 hasConceptScore W2068087647C143065580 @default.
• W2068087647 hasConceptScore W2068087647C147816474 @default.
• W2068087647 hasConceptScore W2068087647C154881586 @default.
• W2068087647 hasConceptScore W2068087647C161790260 @default.
• W2068087647 hasConceptScore W2068087647C178790620 @default.
• W2068087647 hasConceptScore W2068087647C181199279 @default.
• W2068087647 hasConceptScore W2068087647C185592680 @default.
• W2068087647 hasConceptScore W2068087647C191503008 @default.
• W2068087647 hasConceptScore W2068087647C21951064 @default.
• W2068087647 hasConceptScore W2068087647C2779201268 @default.
• W2068087647 hasConceptScore W2068087647C521977710 @default.
• W2068087647 hasConceptScore W2068087647C55493867 @default.
• W2068087647 hasConceptScore W2068087647C9418097 @default.
• W2068087647 hasConceptScore W2068087647C94412978 @default.
• W2068087647 hasLocation W20680876471 @default.
• W2068087647 hasLocation W20680876472 @default.
• W2068087647 hasLocation W20680876473 @default.
• W2068087647 hasLocation W20680876474 @default.
• W2068087647 hasLocation W20680876475 @default.
• W2068087647 hasLocation W20680876476 @default.
• W2068087647 hasOpenAccess W2068087647 @default.
• W2068087647 hasPrimaryLocation W20680876471 @default.
• W2068087647 hasRelatedWork W1503397865 @default.
• W2068087647 hasRelatedWork W1983682392 @default.
• W2068087647 hasRelatedWork W2076143662 @default.
• W2068087647 hasRelatedWork W2088429780 @default.
• W2068087647 hasRelatedWork W2394960306 @default.
• W2068087647 hasRelatedWork W2527725383 @default.
• W2068087647 hasRelatedWork W2888815005 @default.
• W2068087647 hasRelatedWork W2907501679 @default.

• next