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• W2068093124 abstract "We tested the in vitro feasibility of porous PCL (poly(ε-caprolactone)) as a scaffold for cartilage tissue engineering from mesenchymal stem cells (MSCs) and determined the effects of various surface treatments. Three porous PCL scaffold modifications were examined: (1) PCL/Pluronic F127, (2) PCL/collagen, and (3) PCL/Pluronic F127/collagen, in addition to (4) PCL-only. MSCs (5 × 105) were seeded in PCL scaffolds of pore size 100–150 μm, and after 3 weeks of in vitro culture, MSC-scaffolds were investigated for gross appearance, DNA amount, glycosaminoglycan (GAG) content, chondrogenic gene expression, and histology. Grossly, the cell-scaffold complexes became harder, and were more easily manipulated with a forceps after 3 weeks of culture. The three surface-treated scaffolds had higher DNA contents than did the PCL-only scaffold, and the GAG contents in PCL/collagen and PCL/F127/collagen scaffolds were higher than those seen in the PCL-only scaffold. Real-time PCR showed that Sox-9 and COL2A1 mRNA levels were remarkably elevated in PCL/collagen and PCL/F127/collagen scaffolds versus the PCL-only scaffold. On the other hand, Col1A1 and Col10A1 mRNA levels were lower in the three modified PCL scaffolds than in the PCL-only scaffold. Histological findings generally concurred with GAG and RT-PCR findings, and demonstrated the affinity of PCL-based scaffolds for MSCs and the potentials of these scaffold to induce chondrogenic differentiation. Cells showed more differentiated appearance and more abundant extracellular matrix formation in PCL/collagen and PCL/collagen/F127 scaffolds. Our findings suggest that PCL-based porous scaffolds may be useful carriers for MSC transplantation in the cartilage tissue engineering field, and that collagen-based surface modifications further enhance the chondrogenic differentiation of MSCs. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010" @default.
• W2068093124 created "2016-06-24" @default.
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• W2068093124 date "2009-01-01" @default.
• W2068093124 modified "2023-10-17" @default.
• W2068093124 title "Chondrogenesis using mesenchymal stem cells and PCL scaffolds" @default.
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• W2068093124 doi "https://doi.org/10.1002/jbm.a.32414" @default.
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