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• W2068122914 startingPage "8440" @default.
• W2068122914 abstract "Although the hydroxyl radical is often implicated as the species responsible for the initiation of oxidative damage in iron-overload conditions, no ESR evidence for the formation of the radical in vivo has been reported. We have employed a secondary radical-trapping technique in which the hydroxyl radical reacts with dimethyl sulfoxide to form the methyl radical, which is then detected as its adduct of the spin trap N-t-butyl-alpha-phenylnitrone in the bile of animals given an intragastric dose of ferrous sulfate. The identity of this adduct was verified by isotope-substitution techniques. We show that unless measures are taken to inactivate the iron excreted in the bile of treated animals, reactions between iron, oxygen, dimethyl sulfoxide, N-t-butyl-alpha-phenylnitrone, and bile components lead to the formation of artifacts during sample collection." @default.
• W2068122914 created "2016-06-24" @default.
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• W2068122914 date "1991-10-01" @default.
• W2068122914 modified "2023-10-04" @default.
• W2068122914 title "Direct evidence for in vivo hydroxyl-radical generation in experimental iron overload: an ESR spin-trapping investigation." @default.
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• W2068122914 doi "https://doi.org/10.1073/pnas.88.19.8440" @default.
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