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• W2068129612 abstract "The coronavirus nucleocapsid protein (N) plays an essential structural role in virions through a network of interactions with positive-strand viral genomic RNA, the envelope membrane protein (M), and other N molecules. Additionally, N protein participates in at least one stage of the complex mechanism of coronavirus RNA synthesis. We previously uncovered an unanticipated interaction between N and the largest subunit of the viral replicase-transcriptase complex, nonstructural protein 3 (nsp3). This was found through analysis of revertants of a severely defective mutant of murine hepatitis virus (MHV) in which the N gene was replaced with that of its close relative, bovine coronavirus (BCoV). In the work reported here, we constructed BCoV chimeras and other mutants of MHV nsp3 and obtained complementary genetic evidence for its association with N protein. We found that the N-nsp3 interaction maps to the amino-terminal ubiquitin-like domain of nsp3, which is essential for the virus. The interaction does not require the adjacent acidic domain of nsp3, which is dispensable. In addition, we demonstrated a complete correspondence between N-nsp3 genetic interactions and the ability of N protein to enhance the infectivity of transfected coronavirus genomic RNA. The latter function of N was shown to depend on both of the RNA-binding domains of N, as well as on the serine- and arginine-rich central region of N, which binds nsp3. Our results support a model in which the N-nsp3 interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection." @default.
• W2068129612 created "2016-06-24" @default.
• W2068129612 creator A5006527362 @default.
• W2068129612 creator A5032598730 @default.
• W2068129612 creator A5079073748 @default.
• W2068129612 date "2013-08-15" @default.
• W2068129612 modified "2023-09-30" @default.
• W2068129612 title "Characterization of a Critical Interaction between the Coronavirus Nucleocapsid Protein and Nonstructural Protein 3 of the Viral Replicase-Transcriptase Complex" @default.
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• W2068129612 doi "https://doi.org/10.1128/jvi.01275-13" @default.
• W2068129612 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3754073" @default.
• W2068129612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23760243" @default.
• W2068129612 hasPublicationYear "2013" @default.
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