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• W2068133732 abstract "Complete isocapnic O2 equilibrium curves (O2EC's) and related blood-gas properties are reported for whole blood of the bullfrog (Rana catesbeiana) and the aquatic turtle (Pseudemys scripta) at temperatures ranging from 5 to 35°C. P50's for bulldog and turtle blood at physiological pH and 25°C were 36.6 Torr (pH 7.83) and 19.3 Torr (pH 7.55), respectively. Elevation of blood temperature significantly reduced hemoglogbin oxygen affinity in both species (ΔH = −8.1 and 7.8 kcal/mol O2 for Rana and Pseudemys, respectively). Bullfrog and turtle oxygen equilibrium data revealed non-standard curve shapes when compared with the Severinghaus curve for human blood (1979); ectotherm O2EC's rose more steeply below P50 (less sigmond) and were distinctly flattened (linear) above 50% saturation. The CO2-Bohr effect for bullfrog and turtle blood varied significantly as a function of saturation. In addtion, both species exhibited non-linear Hill relationships (logS/1-S vs. logPO2. These results indicate that oxygen binding properties of the multiple hemoglobin bloods of Rana and Pseudemys (demonstrated by isoelectric focusing) are more complex than those exhibited by normal human blood. As a consequence, these extotherm blood oxygen data are not well characterized by the limited number of simple descriptive parameters (P50, Hill's n and ΔlogP50/ΔpH) commonly used to delineate predominantely single hemoglobin systems." @default.
• W2068133732 created "2016-06-24" @default.
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• W2068133732 date "1980-12-01" @default.
• W2068133732 modified "2023-09-28" @default.
• W2068133732 title "Oxygen equilibria of ectotherm blood containing multiple hemoglobins" @default.
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• W2068133732 doi "https://doi.org/10.1016/0034-5687(80)90123-1" @default.
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