FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068151325> ?p ?o ?g. }

• W2068151325 endingPage "1241" @default.
• W2068151325 startingPage "1235" @default.
• W2068151325 abstract "Remifentanil is a μ-opioid receptor agonist that was developed as a synthetic opioid for use in anesthesia and intensive care medicine. Remifentanil is rapidly metabolized in both blood and tissues, which results in a very short duration of action. Even after blood sampling, remifentanil is unstable in whole blood and plasma through endogenous esterases and chemical hydrolysis. The instability of remifentanil in these matrices makes sample collection and processing a critical phase in the bioanalysis of remifentanil.We have developed a fast and simple sample preparation method using protein precipitation followed by liquid chromatography-tandem mass spectrometry analysis. To improve the stability of remifentanil, citric acid, ascorbic acid, and formic acid were investigated for acidification of EDTA plasma. The stability of remifentanil was investigated in stock solution, EDTA whole blood, EDTA plasma, and acidified EDTA plasma at ambient temperature, 4 °C, 0 °C, and at -20 °C.The analytical method was fully validated based on the Food and Drug Administration guidelines for bioanalytical method validation with a large linear range of 0.20 to 250 ng/mL remifentanil in EDTA plasma acidified with formic acid. The stability results of remifentanil in EDTA tubes, containing whole blood placed in ice water, showed a decrease of approximately 2% in 2 hours. EDTA plasma acidified with citric acid, formic acid, and ascorbic acid showed 0.5%, 4.2%, and 7.2% remifentanil degradation, respectively, after 19 hours at ambient temperature. Formic acid was chosen because of its volatility and thus liquid chromatography-tandem mass spectrometry compatibility. The use of formic acid added to EDTA plasma improved the stability of remifentanil, which was stable for 2 days at ambient temperature, 14 days at 4 °C, and 103 days at -20 °C.The analytical method we developed uses a simple protein precipitation and maximal throughput by a 2-point calibration curve and short run times of 2.6 minutes. Best sample stability is obtained by placing tubes containing EDTA whole blood in ice water directly after sampling, followed by centrifugation and transfer of the EDTA plasma to tubes with formic acid. The stability of remifentanil in EDTA plasma was significantly improved by the addition of 1.5 μL formic acid per milliliter of EDTA plasma. This analytical method and sample pretreatment are suitable for remifentanil pharmacokinetic studies." @default.
• W2068151325 created "2016-06-24" @default.
• W2068151325 creator A5002946533 @default.
• W2068151325 creator A5004578527 @default.
• W2068151325 creator A5018163150 @default.
• W2068151325 creator A5048910956 @default.
• W2068151325 creator A5050978066 @default.
• W2068151325 creator A5062373450 @default.
• W2068151325 date "2015-06-01" @default.
• W2068151325 modified "2023-10-14" @default.
• W2068151325 title "Analysis of Remifentanil with Liquid Chromatography-Tandem Mass Spectrometry and an Extensive Stability Investigation in EDTA Whole Blood and Acidified EDTA Plasma" @default.
• W2068151325 cites W1965121445 @default.
• W2068151325 cites W1974231876 @default.
• W2068151325 cites W1976604622 @default.
• W2068151325 cites W1979916930 @default.
• W2068151325 cites W1998598553 @default.
• W2068151325 cites W2024687868 @default.
• W2068151325 cites W2050282468 @default.
• W2068151325 cites W2055449236 @default.
• W2068151325 cites W2064676985 @default.
• W2068151325 cites W2067344915 @default.
• W2068151325 cites W2078432529 @default.
• W2068151325 cites W2089539732 @default.
• W2068151325 cites W2122833898 @default.
• W2068151325 cites W2165086894 @default.
• W2068151325 cites W2488700677 @default.
• W2068151325 doi "https://doi.org/10.1213/ane.0000000000000643" @default.
• W2068151325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25692453" @default.
• W2068151325 hasPublicationYear "2015" @default.
• W2068151325 type Work @default.
• W2068151325 sameAs 2068151325 @default.
• W2068151325 citedByCount "13" @default.
• W2068151325 countsByYear W20681513252017 @default.
• W2068151325 countsByYear W20681513252018 @default.
• W2068151325 countsByYear W20681513252019 @default.
• W2068151325 countsByYear W20681513252020 @default.
• W2068151325 countsByYear W20681513252021 @default.
• W2068151325 countsByYear W20681513252023 @default.
• W2068151325 crossrefType "journal-article" @default.
• W2068151325 hasAuthorship W2068151325A5002946533 @default.
• W2068151325 hasAuthorship W2068151325A5004578527 @default.
• W2068151325 hasAuthorship W2068151325A5018163150 @default.
• W2068151325 hasAuthorship W2068151325A5048910956 @default.
• W2068151325 hasAuthorship W2068151325A5050978066 @default.
• W2068151325 hasAuthorship W2068151325A5062373450 @default.
• W2068151325 hasBestOaLocation W20681513251 @default.
• W2068151325 hasConcept C162356407 @default.
• W2068151325 hasConcept C185592680 @default.
• W2068151325 hasConcept C2776277131 @default.
• W2068151325 hasConcept C2777379063 @default.
• W2068151325 hasConcept C2778891214 @default.
• W2068151325 hasConcept C2779032678 @default.
• W2068151325 hasConcept C2986274086 @default.
• W2068151325 hasConcept C31827203 @default.
• W2068151325 hasConcept C31903555 @default.
• W2068151325 hasConcept C42219234 @default.
• W2068151325 hasConcept C43617362 @default.
• W2068151325 hasConcept C55493867 @default.
• W2068151325 hasConcept C64489805 @default.
• W2068151325 hasConcept C71924100 @default.
• W2068151325 hasConcept C74774607 @default.
• W2068151325 hasConceptScore W2068151325C162356407 @default.
• W2068151325 hasConceptScore W2068151325C185592680 @default.
• W2068151325 hasConceptScore W2068151325C2776277131 @default.
• W2068151325 hasConceptScore W2068151325C2777379063 @default.
• W2068151325 hasConceptScore W2068151325C2778891214 @default.
• W2068151325 hasConceptScore W2068151325C2779032678 @default.
• W2068151325 hasConceptScore W2068151325C2986274086 @default.
• W2068151325 hasConceptScore W2068151325C31827203 @default.
• W2068151325 hasConceptScore W2068151325C31903555 @default.
• W2068151325 hasConceptScore W2068151325C42219234 @default.
• W2068151325 hasConceptScore W2068151325C43617362 @default.
• W2068151325 hasConceptScore W2068151325C55493867 @default.
• W2068151325 hasConceptScore W2068151325C64489805 @default.
• W2068151325 hasConceptScore W2068151325C71924100 @default.
• W2068151325 hasConceptScore W2068151325C74774607 @default.
• W2068151325 hasIssue "6" @default.
• W2068151325 hasLocation W20681513251 @default.
• W2068151325 hasLocation W20681513252 @default.
• W2068151325 hasLocation W20681513253 @default.
• W2068151325 hasLocation W20681513254 @default.
• W2068151325 hasLocation W20681513255 @default.
• W2068151325 hasOpenAccess W2068151325 @default.
• W2068151325 hasPrimaryLocation W20681513251 @default.
• W2068151325 hasRelatedWork W1571490649 @default.
• W2068151325 hasRelatedWork W1976676970 @default.
• W2068151325 hasRelatedWork W1986005472 @default.
• W2068151325 hasRelatedWork W2008473017 @default.
• W2068151325 hasRelatedWork W2011807573 @default.
• W2068151325 hasRelatedWork W2079882259 @default.
• W2068151325 hasRelatedWork W2158180677 @default.
• W2068151325 hasRelatedWork W2803395174 @default.
• W2068151325 hasRelatedWork W2889281564 @default.
• W2068151325 hasRelatedWork W2936345590 @default.
• W2068151325 hasVolume "120" @default.
• W2068151325 isParatext "false" @default.
• W2068151325 isRetracted "false" @default.
• W2068151325 magId "2068151325" @default.

• next