FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068180122> ?p ?o ?g. }

• W2068180122 endingPage "74" @default.
• W2068180122 startingPage "66" @default.
• W2068180122 abstract "With increasing application of Hansenula polymorpha in fundamental research and biotechnology, many more genetic manipulations are required. However, these have been restricted for the finiteness of selectable markers. Here, MazF, a toxin protein from Escherichia coli, was investigated as a counter-selectable marker in H. polymorpha. The lethal effect of MazF on yeast cells suggested that it is a candidate for counter-selection in H. polymorpha. Markerless or scarless gene deletion in H. polymorpha was conducted based on selectable markers cassette mazF-zeoR, in which the zeocin resistance cassette and mazF expression cassette were used as positive and counter-selectable markers, respectively. For markerless deletion, the target region can be replaced by CYC1TT via two-step homologous recombination. For scarless deletion, the innate upstream region (5′UP) of target genes rather than CYC1TT mediates homologous recombination to excise both selectable markers and 5′ sequence of target genes. Moreover, scarless deletion can be accomplished by using short homologous arms for the effectiveness of mazF as a counter-selectable marker. The applicability of the strategies in markerless or scarless deletion of PEP4, LEU2, and TRP1 indicates that this study provides easy, time-efficient, and host-independent protocols for single or multiple genetic manipulations in H. polymorpha." @default.
• W2068180122 created "2016-06-24" @default.
• W2068180122 creator A5022551930 @default.
• W2068180122 creator A5028211408 @default.
• W2068180122 creator A5029718309 @default.
• W2068180122 creator A5038631661 @default.
• W2068180122 creator A5052925180 @default.
• W2068180122 creator A5055809196 @default.
• W2068180122 date "2015-01-01" @default.
• W2068180122 modified "2023-10-14" @default.
• W2068180122 title "Scarless gene deletion in methylotrophic Hansenula polymorpha by using mazF as counter-selectable marker" @default.
• W2068180122 cites W1559808711 @default.
• W2068180122 cites W1585624241 @default.
• W2068180122 cites W1966712692 @default.
• W2068180122 cites W1971401041 @default.
• W2068180122 cites W1997007523 @default.
• W2068180122 cites W2005266650 @default.
• W2068180122 cites W2008407605 @default.
• W2068180122 cites W2012173628 @default.
• W2068180122 cites W2024365710 @default.
• W2068180122 cites W2024969758 @default.
• W2068180122 cites W2034498656 @default.
• W2068180122 cites W2038364847 @default.
• W2068180122 cites W2051056772 @default.
• W2068180122 cites W2052497254 @default.
• W2068180122 cites W2058869872 @default.
• W2068180122 cites W2078170397 @default.
• W2068180122 cites W2078852372 @default.
• W2068180122 cites W2083873972 @default.
• W2068180122 cites W2100760884 @default.
• W2068180122 cites W2107185990 @default.
• W2068180122 cites W2108278134 @default.
• W2068180122 cites W2113633071 @default.
• W2068180122 cites W2119111999 @default.
• W2068180122 cites W2122012609 @default.
• W2068180122 cites W2126429400 @default.
• W2068180122 cites W2127398938 @default.
• W2068180122 cites W2150501344 @default.
• W2068180122 cites W2153992408 @default.
• W2068180122 cites W2163615393 @default.
• W2068180122 cites W2165630230 @default.
• W2068180122 cites W4239882984 @default.
• W2068180122 doi "https://doi.org/10.1016/j.ab.2014.09.006" @default.
• W2068180122 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25233001" @default.
• W2068180122 hasPublicationYear "2015" @default.
• W2068180122 type Work @default.
• W2068180122 sameAs 2068180122 @default.
• W2068180122 citedByCount "8" @default.
• W2068180122 countsByYear W20681801222016 @default.
• W2068180122 countsByYear W20681801222018 @default.
• W2068180122 countsByYear W20681801222019 @default.
• W2068180122 countsByYear W20681801222021 @default.
• W2068180122 countsByYear W20681801222022 @default.
• W2068180122 crossrefType "journal-article" @default.
• W2068180122 hasAuthorship W2068180122A5022551930 @default.
• W2068180122 hasAuthorship W2068180122A5028211408 @default.
• W2068180122 hasAuthorship W2068180122A5029718309 @default.
• W2068180122 hasAuthorship W2068180122A5038631661 @default.
• W2068180122 hasAuthorship W2068180122A5052925180 @default.
• W2068180122 hasAuthorship W2068180122A5055809196 @default.
• W2068180122 hasConcept C100012643 @default.
• W2068180122 hasConcept C102744134 @default.
• W2068180122 hasConcept C104317684 @default.
• W2068180122 hasConcept C22744801 @default.
• W2068180122 hasConcept C40767141 @default.
• W2068180122 hasConcept C42411736 @default.
• W2068180122 hasConcept C54355233 @default.
• W2068180122 hasConcept C547475151 @default.
• W2068180122 hasConcept C64894306 @default.
• W2068180122 hasConcept C70721500 @default.
• W2068180122 hasConcept C86803240 @default.
• W2068180122 hasConcept C92087593 @default.
• W2068180122 hasConceptScore W2068180122C100012643 @default.
• W2068180122 hasConceptScore W2068180122C102744134 @default.
• W2068180122 hasConceptScore W2068180122C104317684 @default.
• W2068180122 hasConceptScore W2068180122C22744801 @default.
• W2068180122 hasConceptScore W2068180122C40767141 @default.
• W2068180122 hasConceptScore W2068180122C42411736 @default.
• W2068180122 hasConceptScore W2068180122C54355233 @default.
• W2068180122 hasConceptScore W2068180122C547475151 @default.
• W2068180122 hasConceptScore W2068180122C64894306 @default.
• W2068180122 hasConceptScore W2068180122C70721500 @default.
• W2068180122 hasConceptScore W2068180122C86803240 @default.
• W2068180122 hasConceptScore W2068180122C92087593 @default.
• W2068180122 hasLocation W20681801221 @default.
• W2068180122 hasLocation W20681801222 @default.
• W2068180122 hasOpenAccess W2068180122 @default.
• W2068180122 hasPrimaryLocation W20681801221 @default.
• W2068180122 hasRelatedWork W1571771606 @default.
• W2068180122 hasRelatedWork W1873683136 @default.
• W2068180122 hasRelatedWork W2074597425 @default.
• W2068180122 hasRelatedWork W2108449047 @default.
• W2068180122 hasRelatedWork W2350652591 @default.
• W2068180122 hasRelatedWork W2524850911 @default.
• W2068180122 hasRelatedWork W2620737981 @default.
• W2068180122 hasRelatedWork W3023578459 @default.
• W2068180122 hasRelatedWork W2092874662 @default.
• W2068180122 hasRelatedWork W2097349615 @default.

• next