FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068206148> ?p ?o ?g. }

• W2068206148 endingPage "231" @default.
• W2068206148 startingPage "221" @default.
• W2068206148 abstract "S-adenosylmethionine (SAMe) and its metabolite 5′-methylthioadenosine (MTA) are proapoptotic in HepG2 cells. In microarray studies, we found SAMe treatment induced Bcl-x expression. Bcl-x is alternatively spliced to produce two distinct mRNAs and proteins, Bcl-xL and Bcl-xS. Bcl-xL is antiapoptotic, while Bcl-xS is proapoptotic. In this study we showed that SAMe and MTA selectively induced Bcl-xS in a time- and dose-dependent manner in HepG2 cells. There are three transcription start sites in the human Bcl-x gene which yield only Bcl-xL in control HepG2 cells. SAMe and MTA treatment did not affect promoter usage, but while one promoter yielded only Bcl-xL, the other two yielded both Bcl-xL and Bcl-xS, with Bcl-xS as the predominant messenger RNA (mRNA) species. Trichostatin A, 3-deaza-adenosine, cycloleucine, and okadaic acid had no effect on Bcl-xS induction by SAMe or MTA. Calyculin A and tautomycin, on the other hand, blocked SAMe and MTA-mediated Bcl-xS induction and apoptosis in a dose-dependent manner. SAMe and MTA increased protein phosphatase 1 (PP1) catalytic subunit mRNA and protein levels and dephosphorylation of serine–arginine proteins, with the latter blocked by calyculin A. The effects of SAMe and MTA on Bcl-xS, PP1 expression, and apoptosis were also seen in 293 cells, but not in primary hepatocytes. Induction of Bcl-xS by ceramide in HepG2 cells also resulted in apoptosis. In conclusion, we have uncovered a highly novel action of SAMe and MTA, namely the ability to affect the cellular phosphorylation state and alternative splicing of genes, in this case resulting in the induction of Bcl-xS leading to apoptosis. Supplementary material for this article can be found on the HEPATOLOGY website (http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html). (HEPATOLOGY 2004;40:221–231.)" @default.
• W2068206148 created "2016-06-24" @default.
• W2068206148 creator A5009884211 @default.
• W2068206148 creator A5010187175 @default.
• W2068206148 creator A5010466371 @default.
• W2068206148 creator A5013928900 @default.
• W2068206148 creator A5020455290 @default.
• W2068206148 creator A5029938378 @default.
• W2068206148 creator A5040166946 @default.
• W2068206148 creator A5062407468 @default.
• W2068206148 creator A5075209582 @default.
• W2068206148 creator A5083359573 @default.
• W2068206148 date "2004-07-01" @default.
• W2068206148 modified "2023-09-27" @default.
• W2068206148 title "S-adenosylmethionine and its metabolite induce apoptosis in HepG2 cells: Role of protein phosphatase 1 and Bcl-x_S" @default.
• W2068206148 cites W1490329277 @default.
• W2068206148 cites W1610329392 @default.
• W2068206148 cites W1967894548 @default.
• W2068206148 cites W1968560682 @default.
• W2068206148 cites W1989327230 @default.
• W2068206148 cites W1990754063 @default.
• W2068206148 cites W1993519463 @default.
• W2068206148 cites W1996892555 @default.
• W2068206148 cites W2003558241 @default.
• W2068206148 cites W2007057690 @default.
• W2068206148 cites W2018404966 @default.
• W2068206148 cites W2026923004 @default.
• W2068206148 cites W2027005520 @default.
• W2068206148 cites W2052452850 @default.
• W2068206148 cites W2063176993 @default.
• W2068206148 cites W2078410199 @default.
• W2068206148 cites W2080738906 @default.
• W2068206148 cites W2088284510 @default.
• W2068206148 cites W2095342924 @default.
• W2068206148 cites W2104277391 @default.
• W2068206148 cites W2105333852 @default.
• W2068206148 cites W2108222684 @default.
• W2068206148 cites W2109585569 @default.
• W2068206148 cites W2113977088 @default.
• W2068206148 cites W2115153548 @default.
• W2068206148 cites W2127281349 @default.
• W2068206148 cites W2128154787 @default.
• W2068206148 cites W2139033181 @default.
• W2068206148 cites W2160173410 @default.
• W2068206148 cites W2317506706 @default.
• W2068206148 cites W4294216491 @default.
• W2068206148 doi "https://doi.org/10.1002/hep.20274" @default.
• W2068206148 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15239106" @default.
• W2068206148 hasPublicationYear "2004" @default.
• W2068206148 type Work @default.
• W2068206148 sameAs 2068206148 @default.
• W2068206148 citedByCount "77" @default.
• W2068206148 countsByYear W20682061482012 @default.
• W2068206148 countsByYear W20682061482013 @default.
• W2068206148 countsByYear W20682061482014 @default.
• W2068206148 countsByYear W20682061482015 @default.
• W2068206148 countsByYear W20682061482017 @default.
• W2068206148 countsByYear W20682061482018 @default.
• W2068206148 countsByYear W20682061482019 @default.
• W2068206148 countsByYear W20682061482020 @default.
• W2068206148 countsByYear W20682061482021 @default.
• W2068206148 crossrefType "journal-article" @default.
• W2068206148 hasAuthorship W2068206148A5009884211 @default.
• W2068206148 hasAuthorship W2068206148A5010187175 @default.
• W2068206148 hasAuthorship W2068206148A5010466371 @default.
• W2068206148 hasAuthorship W2068206148A5013928900 @default.
• W2068206148 hasAuthorship W2068206148A5020455290 @default.
• W2068206148 hasAuthorship W2068206148A5029938378 @default.
• W2068206148 hasAuthorship W2068206148A5040166946 @default.
• W2068206148 hasAuthorship W2068206148A5062407468 @default.
• W2068206148 hasAuthorship W2068206148A5075209582 @default.
• W2068206148 hasAuthorship W2068206148A5083359573 @default.
• W2068206148 hasBestOaLocation W20682061481 @default.
• W2068206148 hasConcept C104317684 @default.
• W2068206148 hasConcept C105580179 @default.
• W2068206148 hasConcept C11960822 @default.
• W2068206148 hasConcept C153911025 @default.
• W2068206148 hasConcept C178666793 @default.
• W2068206148 hasConcept C181912034 @default.
• W2068206148 hasConcept C190283241 @default.
• W2068206148 hasConcept C2776953658 @default.
• W2068206148 hasConcept C2779408958 @default.
• W2068206148 hasConcept C55493867 @default.
• W2068206148 hasConcept C86803240 @default.
• W2068206148 hasConcept C95444343 @default.
• W2068206148 hasConceptScore W2068206148C104317684 @default.
• W2068206148 hasConceptScore W2068206148C105580179 @default.
• W2068206148 hasConceptScore W2068206148C11960822 @default.
• W2068206148 hasConceptScore W2068206148C153911025 @default.
• W2068206148 hasConceptScore W2068206148C178666793 @default.
• W2068206148 hasConceptScore W2068206148C181912034 @default.
• W2068206148 hasConceptScore W2068206148C190283241 @default.
• W2068206148 hasConceptScore W2068206148C2776953658 @default.
• W2068206148 hasConceptScore W2068206148C2779408958 @default.
• W2068206148 hasConceptScore W2068206148C55493867 @default.
• W2068206148 hasConceptScore W2068206148C86803240 @default.
• W2068206148 hasConceptScore W2068206148C95444343 @default.
• W2068206148 hasIssue "1" @default.

• next