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• W2068263902 abstract "This paper describes a method for the transfer to plant cells of any cloned gene, regardless of its termini or internal restriction enzyme cleavage sites. A broad host-range intermediate vector, pGV1117, was constructed containing HindIII-23, a right-end T-region fragment of the nopaline plasmid pTiC58. Using in vivo protection by EcoRI methylase and EcoRI linker ligation, a fragment of rabbit chromosomal DNA, carrying the β-globin gene, was inserted into plasmid pGV1117. Following transmission to Agrobacterium tumefaciens, insertion of the gene into the T-region of pTiC58 occurred via in vivo recombination. Infection of axenic tobacco seedlings resulted in the transfer to the plant genome of an intact β-globin gene, as part of the T-DNA. Although the gene was stably maintained during tissue culture, β-globin-specific transcripts were not detected in the transformed plant cells." @default.
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• W2068263902 date "1983-09-01" @default.
• W2068263902 modified "2023-10-16" @default.
• W2068263902 title "A general method for the transfer of cloned genes to plant cells" @default.
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• W2068263902 doi "https://doi.org/10.1016/0378-1119(83)90021-5" @default.
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