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- W2068272911 abstract "Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FLThe cancer antigen 125 (CA125) is encoded by the MUC16 gene, which is present in sera of women with ovarian cancer. Currently, CA125 is widely used to diagnose the epithelial ovarian cancer and monitor the effects of treatment. Despite the widespread use of CA125, the biochemical and molecular functions of this antigen is poorly understood. There is no phenotype for MUC16 knockout mice (Cheon et al., PLoS ONE, 2009). Our previous data have demonstrated that the carboxyl-terminus of MUC16 has at least the partial functions of MUC16 since CA125 protein is too big (up to 2.5 MDa). To better understanding of the biological functions and characterizations of MUC16, we made a construct to create a conditional transgenic mouse model for the carboxyl-terminus of MUC16 using the Cre-loxP system. This mouse model encodes for the specific portion of MUC16, we named as MUC16ΔC354 mouse, which includes 354 amino acids of carboxyl-terminus of MUC16 with a HA tag. The CMV early enhancer plus chicken β actin (CAG) promoter is used to drive hGFP between two loxPs and the carboxyl-terminus of MUC16 after removing hGFP. After mating with EIIa-Cre transgenic mice, we found two founders which highly express MUC16ΔC354 in many organs, for example, brain, colon, heart, kidney, liver, lung, ovary and spleen. Lung and heart have higher expression level of MUC16ΔC354, and ovary has a middle level. MUC16ΔC354 heterozygous animals appear to be viable, fertile, and develop normally through the first four months of life. However, MUC16ΔC354 transgenic mice regulate PI3K/AKT and MAPK/ERK signaling pathways in some organs. Additional MUC16ΔC354 mice are being bred and selected for phenotypes. Cross breeding with N-Myc transgenic mice, or Rb-1 and p53 knockout mice is planned based on the preliminary results of The Cancer Genome Atlas (TCGA).Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4332. doi:10.1158/1538-7445.AM2011-4332" @default.
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- W2068272911 date "2011-04-15" @default.
- W2068272911 modified "2023-09-29" @default.
- W2068272911 title "Abstract 4332: Conditional transgenic mice for the carboxyl-terminus of MUC16" @default.
- W2068272911 doi "https://doi.org/10.1158/1538-7445.am2011-4332" @default.
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