FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068275714> ?p ?o ?g. }

• W2068275714 abstract "Pemphigus vulgaris (PV) is a severe autoimmune blistering disease associated with autoantibodies to desmoglein 3 (Dsg 3), a transmembrane glycoprotein of the cadherin family. Previous studies mainly focused on the mapping of conformational epitopes of Dsg 3 using recombinant fragments of Dsg 3 and competition ELISA.Here, we performed a mapping of linear B cell epitopes on Dsg 3 in PV patients by the use of overlapping synthetic peptides.A set of 254 overlapping synthetic peptides of 14 amino acids length covering the entire Dsg 3 extracellular domain was generated. Sera of patients with active PV (n=10) and healthy volunteers (n=10) were tested for IgG reactivity with the 254 peptides by ELISA. Testing each peptide separately, 7 major antigenic sites were identified. In order to validate these reactivities, 7 corresponding peptides of 13-33 amino acids in length were generated and employed by ELISA. Additional sera of active PV patients (n=17) and healthy volunteers (n=20) were tested and the most reactive peptide was used to specifically purify anti-Dsg 3 antibodies from PV sera (n=3).The major autoantibody reactivity in PV sera was mapped to amino acids 333-356 within the EC3 domain. Purifying patients IgG using the identified peptide, however, failed to induce acantholysis in keratinocyte dissociation assay.We conclude that linear epitopes do not play a major pathogenic role in human PV." @default.
• W2068275714 created "2016-06-24" @default.
• W2068275714 creator A5001914009 @default.
• W2068275714 creator A5008159107 @default.
• W2068275714 creator A5021559096 @default.
• W2068275714 creator A5024206866 @default.
• W2068275714 creator A5056292574 @default.
• W2068275714 creator A5067198663 @default.
• W2068275714 creator A5081165613 @default.
• W2068275714 creator A5090549024 @default.
• W2068275714 date "2012-02-01" @default.
• W2068275714 modified "2023-09-27" @default.
• W2068275714 title "Mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides" @default.
• W2068275714 cites W1602264889 @default.
• W2068275714 cites W1927348210 @default.
• W2068275714 cites W1965507945 @default.
• W2068275714 cites W1966272332 @default.
• W2068275714 cites W1971386513 @default.
• W2068275714 cites W1976222207 @default.
• W2068275714 cites W1985278228 @default.
• W2068275714 cites W1989166114 @default.
• W2068275714 cites W1991125590 @default.
• W2068275714 cites W2005111877 @default.
• W2068275714 cites W2015590404 @default.
• W2068275714 cites W2019037591 @default.
• W2068275714 cites W2035040893 @default.
• W2068275714 cites W2040189845 @default.
• W2068275714 cites W2041827428 @default.
• W2068275714 cites W2045090980 @default.
• W2068275714 cites W2046534727 @default.
• W2068275714 cites W2057784301 @default.
• W2068275714 cites W2057812542 @default.
• W2068275714 cites W2058595201 @default.
• W2068275714 cites W2059966853 @default.
• W2068275714 cites W2077618466 @default.
• W2068275714 cites W2079704653 @default.
• W2068275714 cites W2088720482 @default.
• W2068275714 cites W2094084130 @default.
• W2068275714 cites W2094800200 @default.
• W2068275714 cites W2097531433 @default.
• W2068275714 cites W2100905754 @default.
• W2068275714 cites W2142068606 @default.
• W2068275714 cites W2147166417 @default.
• W2068275714 cites W2157037806 @default.
• W2068275714 cites W2160684214 @default.
• W2068275714 cites W2171300323 @default.
• W2068275714 cites W2429086420 @default.
• W2068275714 doi "https://doi.org/10.1016/j.jdermsci.2011.11.012" @default.
• W2068275714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22261006" @default.
• W2068275714 hasPublicationYear "2012" @default.
• W2068275714 type Work @default.
• W2068275714 sameAs 2068275714 @default.
• W2068275714 citedByCount "9" @default.
• W2068275714 countsByYear W20682757142013 @default.
• W2068275714 countsByYear W20682757142014 @default.
• W2068275714 countsByYear W20682757142015 @default.
• W2068275714 countsByYear W20682757142016 @default.
• W2068275714 countsByYear W20682757142018 @default.
• W2068275714 crossrefType "journal-article" @default.
• W2068275714 hasAuthorship W2068275714A5001914009 @default.
• W2068275714 hasAuthorship W2068275714A5008159107 @default.
• W2068275714 hasAuthorship W2068275714A5021559096 @default.
• W2068275714 hasAuthorship W2068275714A5024206866 @default.
• W2068275714 hasAuthorship W2068275714A5056292574 @default.
• W2068275714 hasAuthorship W2068275714A5067198663 @default.
• W2068275714 hasAuthorship W2068275714A5081165613 @default.
• W2068275714 hasAuthorship W2068275714A5090549024 @default.
• W2068275714 hasConcept C104317684 @default.
• W2068275714 hasConcept C147483822 @default.
• W2068275714 hasConcept C153911025 @default.
• W2068275714 hasConcept C159654299 @default.
• W2068275714 hasConcept C163764329 @default.
• W2068275714 hasConcept C185592680 @default.
• W2068275714 hasConcept C195616568 @default.
• W2068275714 hasConcept C203014093 @default.
• W2068275714 hasConcept C2779281246 @default.
• W2068275714 hasConcept C2780632150 @default.
• W2068275714 hasConcept C2780684067 @default.
• W2068275714 hasConcept C2781241564 @default.
• W2068275714 hasConcept C2781313415 @default.
• W2068275714 hasConcept C2909223747 @default.
• W2068275714 hasConcept C40767141 @default.
• W2068275714 hasConcept C515207424 @default.
• W2068275714 hasConcept C55493867 @default.
• W2068275714 hasConcept C86803240 @default.
• W2068275714 hasConceptScore W2068275714C104317684 @default.
• W2068275714 hasConceptScore W2068275714C147483822 @default.
• W2068275714 hasConceptScore W2068275714C153911025 @default.
• W2068275714 hasConceptScore W2068275714C159654299 @default.
• W2068275714 hasConceptScore W2068275714C163764329 @default.
• W2068275714 hasConceptScore W2068275714C185592680 @default.
• W2068275714 hasConceptScore W2068275714C195616568 @default.
• W2068275714 hasConceptScore W2068275714C203014093 @default.
• W2068275714 hasConceptScore W2068275714C2779281246 @default.
• W2068275714 hasConceptScore W2068275714C2780632150 @default.
• W2068275714 hasConceptScore W2068275714C2780684067 @default.
• W2068275714 hasConceptScore W2068275714C2781241564 @default.
• W2068275714 hasConceptScore W2068275714C2781313415 @default.
• W2068275714 hasConceptScore W2068275714C2909223747 @default.
• W2068275714 hasConceptScore W2068275714C40767141 @default.

• next