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- W2068277781 abstract "A key mechanism for loading acetylcholine (ACh(+)) into synaptic vesicles uses energy to transport H(+) into the vesicle interior and then exchanges H(+) for ACh(+). This mechanism requires anions to follow the H(+) into the vesicles to prevent the building up of an overwhelming electrical gradient across the vesicle membrane. Frog nerve-muscle preparations were treated with hypertonic solution in which sodium gluconate was the major constituent, which substantially increases the sizes of the quanta by increasing their ACh(+) content. The Cl(-) channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) antagonized the increases in quantal size, so it seems likely that Cl(-) follows H(+) to prevent the buildup of a potential gradient across the vesicular membrane." @default.
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- W2068277781 date "2003-01-01" @default.
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- W2068277781 title "A chloride channel blocker reduces acetylcholine uptake into synaptic vesicles at the frog neuromuscular junction" @default.
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- W2068277781 doi "https://doi.org/10.1016/s0006-8993(02)03954-9" @default.
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