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- W2068280750 abstract "A comparative study was made of different methods to determine phytoplankton composition and biomass distribution at 5 stations in the eastern Atlantic Ocean (11° to 35°N). The methods used include plant-pigment analysis and flow cytometry (size class, chlorophyll fluorescence, and phytoplankton DNA distribution). At all 5 stations a Deep Chlorophyll Maximum (DCM) at depths ranging from 80 to 130 m. As indicated by chlorophyll-a2 content, Prochlorococcus sp. made up 25–60% of the total chlorophyll-a biomass in the euphotic zone. However, in terms of relative phytoplankton DNA and carbon content or cellular chlorophyll fluorescence, much higher values were found (45–70%) for this algal species. Prochlorococcus dominated the surface waters, but the eukaryotic phytoplankton component showed the highest biomass values at the DCM and deeper. Analysis of the accessory pigments (applying CHEMTAX) showed a clear vertical gradient at each station, but also the spatial distribution of the different taxonomic classes varied. Flow cytometric analysis of the water samples gave far less detailed information on the species/taxonomical classes present. Classification at the species level was possible only for Prochlorococcus and Synechococcus. In the case of the eukaryotes, the resolution of composition of the two main subpopulations (pico- and small eukaryotes) was enhanced after a DNA specific dye was applied. Staining the genome of the phytoplankton community revealed in most cases 5–15 separable DNA peaks, representing a similar number of different species. In general only a maximum of three DNA peaks dominated (>500 cells per ml). Phytoplankton carbon estimates based on DNA content matched those based on size fractionating filtration and conversion of cell size into carbon. Phytoplankton carbon data combined with the pigment data were used to estimate the carbon to chlorophyll ratios (θ) across the light gradient for a single species (Prochlorococcus) and the eukaryotic phytoplankton community. Because of the increase in chlorophyll-a2 content by a factor 35, the carbon to chlorophyll ratio (θ) of Prochlorococcus showed a fairly uniform pattern with a steep gradient, ranging from a maximum of 450 at the surface to 15 μg C/μg chlor. at 150 m. Furthermore, there was a south to north increase in θ by a factor of two mainly due to lower pigment values per cell of Prochlorococcus in the southern region. In contrast the θ of the collective eukaryotic phytoplankton community showed much lower values at the surface (30–80 μg C/μg chlor.), and varied with depth were far less than did θ of Prochlorococcus (only 3–7 fold variation). The reduced variability in θ of the eukaryotes can be explained by a co-variation of pigmentation and cell size. The latter can be derived from the increase in DNA content per cell with depth. Moreover, both parameters showed that the trend towards a dominance of larger species with declining growth irradiance is accompanied by a shift in community structure. This response differs greatly from that of Prochlorococcus, which tends to possess a high degree of photoacclimation enabling a single species to cover the whole euphotic zone." @default.
- W2068280750 created "2016-06-24" @default.
- W2068280750 creator A5056418648 @default.
- W2068280750 creator A5059212089 @default.
- W2068280750 date "2004-04-01" @default.
- W2068280750 modified "2023-10-08" @default.
- W2068280750 title "Phytoplankton in the subtropical Atlantic Ocean: towards a better assessment of biomass and composition" @default.
- W2068280750 cites W1500942813 @default.
- W2068280750 cites W1560954028 @default.
- W2068280750 cites W1561745237 @default.
- W2068280750 cites W1576973480 @default.
- W2068280750 cites W1602534227 @default.
- W2068280750 cites W1644507360 @default.
- W2068280750 cites W1893723051 @default.
- W2068280750 cites W1965180457 @default.
- W2068280750 cites W1965443778 @default.
- W2068280750 cites W1965757732 @default.
- W2068280750 cites W1968434277 @default.
- W2068280750 cites W1970193008 @default.
- W2068280750 cites W1976826100 @default.
- W2068280750 cites W1979638379 @default.
- W2068280750 cites W1981202205 @default.
- W2068280750 cites W1981674887 @default.
- W2068280750 cites W1981859032 @default.
- W2068280750 cites W1983914643 @default.
- W2068280750 cites W1985200237 @default.
- W2068280750 cites W1986580580 @default.
- W2068280750 cites W1988392661 @default.
- W2068280750 cites W1989981983 @default.
- W2068280750 cites W1994279687 @default.
- W2068280750 cites W2000315305 @default.
- W2068280750 cites W2006959294 @default.
- W2068280750 cites W2013581855 @default.
- W2068280750 cites W2013927815 @default.
- W2068280750 cites W2014469718 @default.
- W2068280750 cites W2014775803 @default.
- W2068280750 cites W2015146119 @default.
- W2068280750 cites W2015180765 @default.
- W2068280750 cites W2020013482 @default.
- W2068280750 cites W2025081245 @default.
- W2068280750 cites W2028101378 @default.
- W2068280750 cites W2028736842 @default.
- W2068280750 cites W2029009657 @default.
- W2068280750 cites W2040558007 @default.
- W2068280750 cites W2041330625 @default.
- W2068280750 cites W2043298706 @default.
- W2068280750 cites W2046152056 @default.
- W2068280750 cites W2046452125 @default.
- W2068280750 cites W2049195872 @default.
- W2068280750 cites W2049706484 @default.
- W2068280750 cites W2050607030 @default.
- W2068280750 cites W2051409771 @default.
- W2068280750 cites W2060460185 @default.
- W2068280750 cites W2065677735 @default.
- W2068280750 cites W2067785566 @default.
- W2068280750 cites W2074732536 @default.
- W2068280750 cites W2079493774 @default.
- W2068280750 cites W2080153486 @default.
- W2068280750 cites W2081106577 @default.
- W2068280750 cites W2082678969 @default.
- W2068280750 cites W2083407589 @default.
- W2068280750 cites W2085615216 @default.
- W2068280750 cites W2087165938 @default.
- W2068280750 cites W2094569713 @default.
- W2068280750 cites W2094572936 @default.
- W2068280750 cites W2094846765 @default.
- W2068280750 cites W2094945194 @default.
- W2068280750 cites W2099132234 @default.
- W2068280750 cites W2102614500 @default.
- W2068280750 cites W2120370089 @default.
- W2068280750 cites W2120702479 @default.
- W2068280750 cites W2123406939 @default.
- W2068280750 cites W2125155327 @default.
- W2068280750 cites W2127328301 @default.
- W2068280750 cites W2135216748 @default.
- W2068280750 cites W2140035359 @default.
- W2068280750 cites W2140119655 @default.
- W2068280750 cites W2141677484 @default.
- W2068280750 cites W2146104126 @default.
- W2068280750 cites W2147629681 @default.
- W2068280750 cites W2149545078 @default.
- W2068280750 cites W2157281339 @default.
- W2068280750 cites W2158062455 @default.
- W2068280750 cites W2158318001 @default.
- W2068280750 cites W2166979580 @default.
- W2068280750 cites W2167598246 @default.
- W2068280750 cites W2167874943 @default.
- W2068280750 doi "https://doi.org/10.1016/j.dsr.2003.12.002" @default.
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