FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068281310> ?p ?o ?g. }

• W2068281310 endingPage "2058" @default.
• W2068281310 startingPage "2050" @default.
• W2068281310 abstract "A variation of affinity capillary electrophoresis, called the replacement ion (RI) method, has been developed to measure the binding of monovalent cations to random sequence, double-stranded (ds) DNA. In this method, the ionic strength is kept constant by gradually replacing a non-binding ion in the solution with a binding ion and measuring the mobility of binding and non-binding analytes as a function of binding ion concentration. The method was validated by measuring the binding of Li+ ions to adenosine nucleotides; the apparent dissociation constants obtained by the RI method are comparable to literature values obtained by other methods. The binding of Tris+, NH4+, Li+, Na+, and K+ to dsDNA was then investigated. The apparent dissociation constants observed for counterion binding to a random-sequence 26-base pair (bp) oligomer ranged from 71 mM for Tris+ to 173 mM for Na+ and K+. Hence, positively charged Tris buffer ions will compete with other monovalent cations in Tris-buffered solutions. The bound cations identified in this study may correspond to the strongly correlated, tightly bound ions recently postulated to exist as a class of ions near the surface of dsDNA (Tan, Z.-J., and Chen, S.-J. (2006) Biophys. J. 91, 518−536). Monovalent cation binding to random-sequence dsDNA would be expected to occur in addition to any site-specific binding of cations to A-tracts or other DNA sequence motifs. Single-stranded DNA oligomers do not bind the five tested cations under the conditions investigated here." @default.
• W2068281310 created "2016-06-24" @default.
• W2068281310 creator A5034359683 @default.
• W2068281310 creator A5077187127 @default.
• W2068281310 creator A5078535393 @default.
• W2068281310 date "2007-01-25" @default.
• W2068281310 modified "2023-10-04" @default.
• W2068281310 title "Quantitative Analysis of Monovalent Counterion Binding to Random-Sequence, Double-Stranded DNA Using the Replacement Ion Method" @default.
• W2068281310 cites W1975797573 @default.
• W2068281310 cites W1979930369 @default.
• W2068281310 cites W1980541251 @default.
• W2068281310 cites W1986579856 @default.
• W2068281310 cites W1986744575 @default.
• W2068281310 cites W1989134705 @default.
• W2068281310 cites W1992370748 @default.
• W2068281310 cites W2000670326 @default.
• W2068281310 cites W2003084716 @default.
• W2068281310 cites W2007761256 @default.
• W2068281310 cites W2012638938 @default.
• W2068281310 cites W2013233309 @default.
• W2068281310 cites W2013714550 @default.
• W2068281310 cites W2026737224 @default.
• W2068281310 cites W2026884660 @default.
• W2068281310 cites W2027914810 @default.
• W2068281310 cites W2030338243 @default.
• W2068281310 cites W2030977104 @default.
• W2068281310 cites W2032514018 @default.
• W2068281310 cites W2033186754 @default.
• W2068281310 cites W2033431596 @default.
• W2068281310 cites W2034815580 @default.
• W2068281310 cites W2038223009 @default.
• W2068281310 cites W2038323867 @default.
• W2068281310 cites W2045056234 @default.
• W2068281310 cites W2052379397 @default.
• W2068281310 cites W2054046276 @default.
• W2068281310 cites W2059720471 @default.
• W2068281310 cites W2059992766 @default.
• W2068281310 cites W2068651731 @default.
• W2068281310 cites W2070224599 @default.
• W2068281310 cites W2070698144 @default.
• W2068281310 cites W2071031034 @default.
• W2068281310 cites W2074262321 @default.
• W2068281310 cites W2075073842 @default.
• W2068281310 cites W2077006735 @default.
• W2068281310 cites W2078629037 @default.
• W2068281310 cites W2079928202 @default.
• W2068281310 cites W2080747920 @default.
• W2068281310 cites W2085296413 @default.
• W2068281310 cites W2095103540 @default.
• W2068281310 cites W2102834112 @default.
• W2068281310 cites W2113339807 @default.
• W2068281310 cites W2117532390 @default.
• W2068281310 cites W2119627230 @default.
• W2068281310 cites W2131578254 @default.
• W2068281310 cites W2135643173 @default.
• W2068281310 cites W2137453899 @default.
• W2068281310 cites W2145126915 @default.
• W2068281310 cites W2154322715 @default.
• W2068281310 cites W2165032708 @default.
• W2068281310 cites W2326919865 @default.
• W2068281310 cites W2512572173 @default.
• W2068281310 cites W4230257048 @default.
• W2068281310 cites W4240558542 @default.
• W2068281310 doi "https://doi.org/10.1021/bi062132w" @default.
• W2068281310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2532990" @default.
• W2068281310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17253778" @default.
• W2068281310 hasPublicationYear "2007" @default.
• W2068281310 type Work @default.
• W2068281310 sameAs 2068281310 @default.
• W2068281310 citedByCount "55" @default.
• W2068281310 countsByYear W20682813102012 @default.
• W2068281310 countsByYear W20682813102013 @default.
• W2068281310 countsByYear W20682813102014 @default.
• W2068281310 countsByYear W20682813102015 @default.
• W2068281310 countsByYear W20682813102016 @default.
• W2068281310 countsByYear W20682813102017 @default.
• W2068281310 countsByYear W20682813102019 @default.
• W2068281310 countsByYear W20682813102021 @default.
• W2068281310 countsByYear W20682813102023 @default.
• W2068281310 crossrefType "journal-article" @default.
• W2068281310 hasAuthorship W2068281310A5034359683 @default.
• W2068281310 hasAuthorship W2068281310A5077187127 @default.
• W2068281310 hasAuthorship W2068281310A5078535393 @default.
• W2068281310 hasBestOaLocation W20682813102 @default.
• W2068281310 hasConcept C102931765 @default.
• W2068281310 hasConcept C107824862 @default.
• W2068281310 hasConcept C145148216 @default.
• W2068281310 hasConcept C147789679 @default.
• W2068281310 hasConcept C170493617 @default.
• W2068281310 hasConcept C178790620 @default.
• W2068281310 hasConcept C184651966 @default.
• W2068281310 hasConcept C185581394 @default.
• W2068281310 hasConcept C185592680 @default.
• W2068281310 hasConcept C187428577 @default.
• W2068281310 hasConcept C2781014771 @default.
• W2068281310 hasConcept C34814092 @default.
• W2068281310 hasConcept C552990157 @default.
• W2068281310 hasConcept C55493867 @default.

• next