Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068283542> ?p ?o ?g. }
- W2068283542 endingPage "142" @default.
- W2068283542 startingPage "131" @default.
- W2068283542 abstract "The nervous system is composed of a wide variety of neurons. A description of the transcriptional profiles of each neuron would yield enormous information about the molecular mechanisms that define morphological or functional characteristics. Here we show that RNA isolation from single neurons is feasible by using an optimized mRNA tagging method. This method extracts transcripts in the target cells by co-immunoprecipitation of the complexes of RNA and epitope-tagged poly(A) binding protein expressed specifically in the cells. With this method and genome-wide microarray, we compared the transcriptional profiles of two functionally different neurons in the main C. elegans gustatory neuron class ASE. Eight of the 13 known subtype-specific genes were successfully detected. Additionally, we identified nine novel genes including a receptor guanylyl cyclase, secreted proteins, a TRPC channel and uncharacterized genes conserved among nematodes, suggesting the two neurons are substantially different than previously thought. The expression of these novel genes was controlled by the previously known regulatory network for subtype differentiation. We also describe unique motif organization within individual gene groups classified by the expression patterns in ASE. Our study paves the way to the complete catalog of the expression profiles of individual C. elegans neurons." @default.
- W2068283542 created "2016-06-24" @default.
- W2068283542 creator A5004168302 @default.
- W2068283542 creator A5025060410 @default.
- W2068283542 creator A5047156095 @default.
- W2068283542 creator A5048325933 @default.
- W2068283542 creator A5050733852 @default.
- W2068283542 date "2009-10-29" @default.
- W2068283542 modified "2023-10-03" @default.
- W2068283542 title "Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans" @default.
- W2068283542 cites W118771962 @default.
- W2068283542 cites W1581412826 @default.
- W2068283542 cites W1944127002 @default.
- W2068283542 cites W1974942735 @default.
- W2068283542 cites W1977630266 @default.
- W2068283542 cites W1985602726 @default.
- W2068283542 cites W2000860920 @default.
- W2068283542 cites W2001481666 @default.
- W2068283542 cites W2003026101 @default.
- W2068283542 cites W2011336267 @default.
- W2068283542 cites W2018507242 @default.
- W2068283542 cites W2022131365 @default.
- W2068283542 cites W2022221238 @default.
- W2068283542 cites W2040805319 @default.
- W2068283542 cites W2051440844 @default.
- W2068283542 cites W2057952525 @default.
- W2068283542 cites W2059130093 @default.
- W2068283542 cites W2065877884 @default.
- W2068283542 cites W2078883869 @default.
- W2068283542 cites W2090149154 @default.
- W2068283542 cites W2094421860 @default.
- W2068283542 cites W2096398908 @default.
- W2068283542 cites W2097396311 @default.
- W2068283542 cites W2102643936 @default.
- W2068283542 cites W2110153008 @default.
- W2068283542 cites W2112253571 @default.
- W2068283542 cites W2114546060 @default.
- W2068283542 cites W2119372697 @default.
- W2068283542 cites W2121777852 @default.
- W2068283542 cites W2122163057 @default.
- W2068283542 cites W2126490785 @default.
- W2068283542 cites W2128016761 @default.
- W2068283542 cites W2144751398 @default.
- W2068283542 cites W2153514402 @default.
- W2068283542 cites W2158366852 @default.
- W2068283542 cites W2160938187 @default.
- W2068283542 cites W2161659751 @default.
- W2068283542 cites W2164693027 @default.
- W2068283542 cites W2165656482 @default.
- W2068283542 cites W2166743396 @default.
- W2068283542 cites W2166760188 @default.
- W2068283542 cites W2171925181 @default.
- W2068283542 cites W2182687083 @default.
- W2068283542 cites W4230501579 @default.
- W2068283542 doi "https://doi.org/10.1093/nar/gkp868" @default.
- W2068283542 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2800226" @default.
- W2068283542 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19875417" @default.
- W2068283542 hasPublicationYear "2009" @default.
- W2068283542 type Work @default.
- W2068283542 sameAs 2068283542 @default.
- W2068283542 citedByCount "28" @default.
- W2068283542 countsByYear W20682835422013 @default.
- W2068283542 countsByYear W20682835422014 @default.
- W2068283542 countsByYear W20682835422015 @default.
- W2068283542 countsByYear W20682835422016 @default.
- W2068283542 countsByYear W20682835422017 @default.
- W2068283542 countsByYear W20682835422018 @default.
- W2068283542 countsByYear W20682835422019 @default.
- W2068283542 countsByYear W20682835422020 @default.
- W2068283542 countsByYear W20682835422021 @default.
- W2068283542 countsByYear W20682835422022 @default.
- W2068283542 crossrefType "journal-article" @default.
- W2068283542 hasAuthorship W2068283542A5004168302 @default.
- W2068283542 hasAuthorship W2068283542A5025060410 @default.
- W2068283542 hasAuthorship W2068283542A5047156095 @default.
- W2068283542 hasAuthorship W2068283542A5048325933 @default.
- W2068283542 hasAuthorship W2068283542A5050733852 @default.
- W2068283542 hasBestOaLocation W20682835421 @default.
- W2068283542 hasConcept C104317684 @default.
- W2068283542 hasConcept C141231307 @default.
- W2068283542 hasConcept C150194340 @default.
- W2068283542 hasConcept C169760540 @default.
- W2068283542 hasConcept C170493617 @default.
- W2068283542 hasConcept C2775908076 @default.
- W2068283542 hasConcept C2778794669 @default.
- W2068283542 hasConcept C2778944004 @default.
- W2068283542 hasConcept C54355233 @default.
- W2068283542 hasConcept C67705224 @default.
- W2068283542 hasConcept C8415881 @default.
- W2068283542 hasConcept C86803240 @default.
- W2068283542 hasConcept C95444343 @default.
- W2068283542 hasConceptScore W2068283542C104317684 @default.
- W2068283542 hasConceptScore W2068283542C141231307 @default.
- W2068283542 hasConceptScore W2068283542C150194340 @default.
- W2068283542 hasConceptScore W2068283542C169760540 @default.
- W2068283542 hasConceptScore W2068283542C170493617 @default.
- W2068283542 hasConceptScore W2068283542C2775908076 @default.
- W2068283542 hasConceptScore W2068283542C2778794669 @default.