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- W2068291288 startingPage "131" @default.
- W2068291288 abstract "The location of nucleosomes on rat satellite I DNA has been investigated using a new approach. Nucleosome cores were prepared from rat liver nuclei with micrococcal nuclease, exonuclease III and nucleases S1. From the total population of core DNA fragments the satellite-containing fragments were isolated by molecular cloning and the complete sequence of 50 clones was determined. The location of nucleosomes along the satellite sequence was found to be non-random. Our results show that nucleosomes occupy a number of positions on satellite I DNA. About 35 to 50% of all nucleosomes are positioned in two corresponding major sites, the remainder in about 16 less preferred sites. The major nucleosome positions are apparently strictly defined with the precision of a single base-pair. These results were confirmed by other approaches, including restriction nuclease digestion experiments. There are good indications of a defined long-range organization of the satellite chromatin fiber in two or more oligonucleosomal arrays with distinct nucleosome configurations." @default.
- W2068291288 created "2016-06-24" @default.
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- W2068291288 date "1984-06-01" @default.
- W2068291288 modified "2023-09-25" @default.
- W2068291288 title "Positioning of nucleosomes in satellite I-containing chromatin of rat liver" @default.
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- W2068291288 doi "https://doi.org/10.1016/0022-2836(84)90385-1" @default.
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