Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068313488> ?p ?o ?g. }
- W2068313488 endingPage "867" @default.
- W2068313488 startingPage "862" @default.
- W2068313488 abstract "The deamination of glutamine is a crucial step in the production of enzymatically hydrolyzed plant proteins to reach high glutamic acid yields. The required glutaminase activity usually is provided by addition of technical enzymes or by in situ generation from fungi, yeast or bacteria (i.e. Aspergillus oryzae in soy sauce production). We screened food-grade Lactobacilli for potential glutaminase activity and selected the enzyme found in Lactobacillus rhamnosus for further characterization. Glutaminase from L. rhamnosus was induced by growing the microorganism on hydrolyzed wheat gluten, a glutamine-rich protein source. Glutamine deaminating activity (glutaminase, EC 3.5.1.2) was found to be membrane-bound and lost its activity gradually upon solubilization. Functional studies of the glutaminase showed an optimal working pH of 7.0 and maximum activity at 50 °C. High salt-tolerance of the enzyme was observed, i.e. the presence of 5% (w/v) salt increased glutaminase activity almost two-fold and 90% of the initial activity still remained at 15% (w/v) salt. The glutaminase activity showed typical Michaelis–Menten behavior with an affinity constant K m of 4.8±0.4 mM for glutamine and a V max of 101±2 U/l." @default.
- W2068313488 created "2016-06-24" @default.
- W2068313488 creator A5007697546 @default.
- W2068313488 creator A5029622683 @default.
- W2068313488 creator A5056032217 @default.
- W2068313488 date "2003-06-01" @default.
- W2068313488 modified "2023-09-25" @default.
- W2068313488 title "Functional characterization of a salt- and thermotolerant glutaminase from Lactobacillus rhamnosus" @default.
- W2068313488 cites W1534362816 @default.
- W2068313488 cites W1603161978 @default.
- W2068313488 cites W1921751707 @default.
- W2068313488 cites W1965386541 @default.
- W2068313488 cites W1982927789 @default.
- W2068313488 cites W1984086156 @default.
- W2068313488 cites W1990708296 @default.
- W2068313488 cites W2008451845 @default.
- W2068313488 cites W2015407678 @default.
- W2068313488 cites W2022583328 @default.
- W2068313488 cites W2027852133 @default.
- W2068313488 cites W2034583757 @default.
- W2068313488 cites W2049117163 @default.
- W2068313488 cites W2065310492 @default.
- W2068313488 cites W2089857389 @default.
- W2068313488 cites W2097608153 @default.
- W2068313488 cites W2155086483 @default.
- W2068313488 cites W2155114427 @default.
- W2068313488 cites W2329952272 @default.
- W2068313488 cites W4256445666 @default.
- W2068313488 doi "https://doi.org/10.1016/s0141-0229(03)00059-0" @default.
- W2068313488 hasPublicationYear "2003" @default.
- W2068313488 type Work @default.
- W2068313488 sameAs 2068313488 @default.
- W2068313488 citedByCount "48" @default.
- W2068313488 countsByYear W20683134882012 @default.
- W2068313488 countsByYear W20683134882013 @default.
- W2068313488 countsByYear W20683134882014 @default.
- W2068313488 countsByYear W20683134882015 @default.
- W2068313488 countsByYear W20683134882016 @default.
- W2068313488 countsByYear W20683134882018 @default.
- W2068313488 countsByYear W20683134882019 @default.
- W2068313488 countsByYear W20683134882020 @default.
- W2068313488 countsByYear W20683134882022 @default.
- W2068313488 countsByYear W20683134882023 @default.
- W2068313488 crossrefType "journal-article" @default.
- W2068313488 hasAuthorship W2068313488A5007697546 @default.
- W2068313488 hasAuthorship W2068313488A5029622683 @default.
- W2068313488 hasAuthorship W2068313488A5056032217 @default.
- W2068313488 hasConcept C100544194 @default.
- W2068313488 hasConcept C109549099 @default.
- W2068313488 hasConcept C181199279 @default.
- W2068313488 hasConcept C185592680 @default.
- W2068313488 hasConcept C198684833 @default.
- W2068313488 hasConcept C2775920511 @default.
- W2068313488 hasConcept C2776503186 @default.
- W2068313488 hasConcept C2776651480 @default.
- W2068313488 hasConcept C2777806559 @default.
- W2068313488 hasConcept C2778541762 @default.
- W2068313488 hasConcept C2778985185 @default.
- W2068313488 hasConcept C2779222958 @default.
- W2068313488 hasConcept C2779349466 @default.
- W2068313488 hasConcept C2780206646 @default.
- W2068313488 hasConcept C2781204770 @default.
- W2068313488 hasConcept C28212737 @default.
- W2068313488 hasConcept C31903555 @default.
- W2068313488 hasConcept C515207424 @default.
- W2068313488 hasConcept C523546767 @default.
- W2068313488 hasConcept C54355233 @default.
- W2068313488 hasConcept C55493867 @default.
- W2068313488 hasConcept C86803240 @default.
- W2068313488 hasConcept C94412978 @default.
- W2068313488 hasConceptScore W2068313488C100544194 @default.
- W2068313488 hasConceptScore W2068313488C109549099 @default.
- W2068313488 hasConceptScore W2068313488C181199279 @default.
- W2068313488 hasConceptScore W2068313488C185592680 @default.
- W2068313488 hasConceptScore W2068313488C198684833 @default.
- W2068313488 hasConceptScore W2068313488C2775920511 @default.
- W2068313488 hasConceptScore W2068313488C2776503186 @default.
- W2068313488 hasConceptScore W2068313488C2776651480 @default.
- W2068313488 hasConceptScore W2068313488C2777806559 @default.
- W2068313488 hasConceptScore W2068313488C2778541762 @default.
- W2068313488 hasConceptScore W2068313488C2778985185 @default.
- W2068313488 hasConceptScore W2068313488C2779222958 @default.
- W2068313488 hasConceptScore W2068313488C2779349466 @default.
- W2068313488 hasConceptScore W2068313488C2780206646 @default.
- W2068313488 hasConceptScore W2068313488C2781204770 @default.
- W2068313488 hasConceptScore W2068313488C28212737 @default.
- W2068313488 hasConceptScore W2068313488C31903555 @default.
- W2068313488 hasConceptScore W2068313488C515207424 @default.
- W2068313488 hasConceptScore W2068313488C523546767 @default.
- W2068313488 hasConceptScore W2068313488C54355233 @default.
- W2068313488 hasConceptScore W2068313488C55493867 @default.
- W2068313488 hasConceptScore W2068313488C86803240 @default.
- W2068313488 hasConceptScore W2068313488C94412978 @default.
- W2068313488 hasIssue "7" @default.
- W2068313488 hasLocation W20683134881 @default.
- W2068313488 hasOpenAccess W2068313488 @default.
- W2068313488 hasPrimaryLocation W20683134881 @default.
- W2068313488 hasRelatedWork W2074495616 @default.