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- W2068364098 abstract "Increased levels of neuropeptide Y correlate with severity of left ventricular hypertrophy in vivo. At cardiomyocyte level, hypertrophy is characterised by increased mass and altered phenotype. The aims were to determine the contributions of increased synthesis and reduced degradation of protein to neuropeptide Y-mediated increase in mass, assess effects on gene expression, and characterise neuropeptide Y Y receptor subtype involvement. Neuropeptide Y (10 nM) increased protein mass of adult rat ventricular cardiomyocytes maintained in culture (24 h) (16%>basal) and de novo protein synthesis (incorporation of [(14)C]phenylalanine) (18%>basal). Neuropeptide Y (100 nM) prevented degradation of existing protein at 8 h. Actinomycin D (5 microM) attenuated increases in protein mass to neuropeptide Y (< or = 1 nM) but not to neuropeptide Y (10 nM). [Leu(31), Pro(34)]neuropeptide Y (10 nM), an agonist at neuropeptide Y Y(1) receptors, increased protein mass (25%>basal) but did not stimulate protein synthesis. Neuropeptide Y-(3-36) (10 nM), an agonist at neuropeptide Y Y(2) receptors, increased protein mass (29%>basal) and increased protein synthesis (13%>basal), respectively. Actinomycin D (5 microM) abolished the increase in protein mass elicited by neuropeptide Y-(3-36) but not that by [Leu(31), Pro(34)]neuropeptide Y. BIBP3226 [(R)-N2-(diphenylacetyl)-N-(4-hydroxyphenylmethyl)-D-arginine amide] (1 microM), a neuropeptide Y Y(1) receptor subtype-selective antagonist, and T(4) [neuropeptide Y-(33-36)](4), a neuropeptide Y Y(2) receptor subtype-selective antagonist, attenuated the increase in protein mass to 100 nM neuropeptide Y by 68% and 59%, respectively. Neuropeptide Y increased expression of the constitutive gene, myosin light chain-2 (MLC-2), maximally at 12 h (4.7-fold>basal) but did not induce (t< or = 36 h) expression of foetal genes (atrial natriuretic peptide (ANP), skeletal-alpha-actin and myosin heavy chain-beta). This increase was attenuated by 86% and 51%, respectively, by BIBP3226 (1 microM) and T(4) [neuropeptide Y-(33-36)](4) (100 nM). [Leu(31), Pro(34)]neuropeptide Y (100 nM) (2.4-fold>basal) and peptide YY-(3-36) (100 nM) (2.3 fold>basal) increased expression of MLC-2 mRNA at 12 h. In conclusion, initiation of cardiomyocyte hypertrophy by neuropeptide Y requires activation of both neuropeptide Y Y(1) and neuropeptide Y Y(2) receptors and is associated with enhanced synthesis and attenuated degradation of protein together with increased expression of constitutive genes but not reinduction of foetal genes." @default.
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- W2068364098 date "2002-04-01" @default.
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- W2068364098 title "Neuropeptide Y Y1 receptor regulates protein turnover and constitutive gene expression in hypertrophying cardiomyocytes" @default.
- W2068364098 cites W1974389718 @default.
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- W2068364098 cites W1982511598 @default.
- W2068364098 cites W1987421901 @default.
- W2068364098 cites W1989248750 @default.
- W2068364098 cites W1990760824 @default.
- W2068364098 cites W2002017388 @default.
- W2068364098 cites W2002962318 @default.
- W2068364098 cites W2003617086 @default.
- W2068364098 cites W2003791055 @default.
- W2068364098 cites W2004646371 @default.
- W2068364098 cites W2005047442 @default.
- W2068364098 cites W2011951708 @default.
- W2068364098 cites W2015524220 @default.
- W2068364098 cites W2020128704 @default.
- W2068364098 cites W2022046088 @default.
- W2068364098 cites W2024245893 @default.
- W2068364098 cites W2026154046 @default.
- W2068364098 cites W2027024239 @default.
- W2068364098 cites W2028645725 @default.
- W2068364098 cites W2028761750 @default.
- W2068364098 cites W2033470721 @default.
- W2068364098 cites W2036569119 @default.
- W2068364098 cites W2041232896 @default.
- W2068364098 cites W2043605142 @default.
- W2068364098 cites W2044583182 @default.
- W2068364098 cites W2048200547 @default.
- W2068364098 cites W2049994320 @default.
- W2068364098 cites W2050251634 @default.
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- W2068364098 cites W2090462942 @default.
- W2068364098 cites W2092743131 @default.
- W2068364098 cites W2103897701 @default.
- W2068364098 cites W2104941760 @default.
- W2068364098 cites W2107785065 @default.
- W2068364098 cites W2131388270 @default.
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- W2068364098 cites W347483702 @default.
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- W2068364098 doi "https://doi.org/10.1016/s0014-2999(02)01440-1" @default.
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