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• W2068385989 abstract "The human neuroblastoma clone SH-SY5Y expresses potassium-, carbachol-, and calcium ionophore A23187-evoked, calcium-dependent release of [3H]noradrenaline. Release in response to carbachol and potassium was greater than additive. Atropine (Ki = 0.33 nM), hexahydrosiladifenidol (Ki = 18 nM), and pirenzepine (Ki = 1,183 nM) completely inhibited the carbachol-evoked noradrenaline release, an order of potency suggesting that an M3 receptor was linked to release. In contrast, noradrenaline release was only partially inhibited by the M2-selective antagonists methoctramine (10(-4) M) and AFDX-116 (10(-4) M), by approximately 14 and 46%, respectively. The nicotinic antagonist d-tubocurarine (10(-4) M) resulted in a partial inhibition of release, a finding suggesting that a nicotinic receptor may also be involved. SH-SY5Y provides a suitable cell line in which to study the biochemical mechanisms underlying the cholinergic receptor regulation of noradrenaline release." @default.
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• W2068385989 date "1991-05-01" @default.
• W2068385989 modified "2023-10-16" @default.
• W2068385989 title "Potassium- and Carbachol-Evoked Release of [³H]Noradrenaline from Human Neuroblastoma Cells, SH-SY5Y" @default.
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• W2068385989 doi "https://doi.org/10.1111/j.1471-4159.1991.tb02085.x" @default.
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