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- W2068391567 abstract "Abstract The aim of this study was to produce stearidonic acid (SDA, 18:4n‐3) omega‐3 concentrates from 25 % SDA soybean oil (SDASO) by enzymatic acidolysis. Substrates were prepared by chemical and enzymatic hydrolysis of SDASO. A 62 % SDA free fatty acid mixture (SDA‐FFA) was obtained by low temperature crystallization of the chemical hydrolyzate while partial hydrolysis of SDASO by non‐immobilized lipase AY 30 ( Candida rugosa ) yielded a 51 % SDA acylglycerol mixture (SDA‐GLY). Reaction conditions for acidolysis between SDA‐FFA and SDA‐GLY were optimized using response surface methodology (RSM). Incorporation of SDA into SDA‐GLY by immobilized Lipozyme RM IM ( Rhizomucor miehei ) and non‐immobilized Lipomod 34P‐LO34P ( C. cylindracea [rugosa] ) lipases were modeled under varying levels of the substrate molar ratio ( S r ), temperature ( T ), and time ( t ). Optimal conditions for production of a 60 % SDA concentrate were predicted to be S r = 4.8, T = 65 °C and t = 8 h for Lipozyme RM IM and S r = 5.0, T = 43 °C and t = 48 h for Lipomod 34P‐L034P. The model was verified experimentally by gram scale synthesis under optimal conditions which resulted in the production of 59.98 and 58.98 % SDA concentrates (≥96 % triacylglycerols) by Lipozyme RM IM and Lipomod 34P‐L034P, respectively." @default.
- W2068391567 created "2016-06-24" @default.
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- W2068391567 date "2013-04-28" @default.
- W2068391567 modified "2023-09-30" @default.
- W2068391567 title "Chemoenzymatic Method for Producing Stearidonic Acid Concentrates from Stearidonic Acid Soybean Oil" @default.
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- W2068391567 doi "https://doi.org/10.1007/s11746-013-2251-y" @default.
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