FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068391719> ?p ?o ?g. }

• W2068391719 endingPage "558" @default.
• W2068391719 startingPage "553" @default.
• W2068391719 abstract "Human cystatin C is a cysteine proteinase inhibitor with potential applications as an anti-viral agent, cancer tumor growth inhibitor, and in prevention of proteolysis during food processing. A glycosylated cystatin C mutant with increased temperature stability was developed for the latter application [Nakamura et al. (1998) FEBS Lett 427:252–254]. A recombinant variant of cystatin C [Nakamura et al. (2000) International patent no. PLTCA99/00717] with two potential sites for N-linked glycosylation was expressed in Pichia pastoris Muts . Little of the cystatin C produced was in the glycosylated form under fermentation conditions of pH 6, temperature 28°C, methanol only feed, and ammonium hydroxide as a nitrogen source. Thus, the effect of addition of complex nitrogen sources, peptone and amino acid supplements, on the yield and glycosylation of this mutant cystatin C were investigated. A full factorial design experiment using 2-l fermenters was performed with three factors: ammonium hydroxide, peptone, and an amino acid mix, at two levels, absent or present. Peptone addition was found to have a positive, and the most significant, effect on cell specific cystatin C yield. A maximum mutant cystatin C yield of 0.82 μmol (g-dry cell weight)−1 min−1 was obtained when all three nitrogen sources were used together. However, under these conditions only 16% of protein was in the glycosylated form since ammonia was found to have a significant negative effect on glycosylation extent. The maximum extent of glycosylation was 30% when peptone and amino acid mix were the only nitrogen sources added." @default.
• W2068391719 created "2016-06-24" @default.
• W2068391719 creator A5066789761 @default.
• W2068391719 creator A5085070545 @default.
• W2068391719 date "2004-11-18" @default.
• W2068391719 modified "2023-09-23" @default.
• W2068391719 title "The effect of nitrogen source on yield and glycosylation of a human cystatin C mutant expressed in Pichia pastoris" @default.
• W2068391719 cites W1485981322 @default.
• W2068391719 cites W1550032963 @default.
• W2068391719 cites W1976658444 @default.
• W2068391719 cites W1979034554 @default.
• W2068391719 cites W1981449476 @default.
• W2068391719 cites W1983866106 @default.
• W2068391719 cites W1984079474 @default.
• W2068391719 cites W1984457499 @default.
• W2068391719 cites W1991890196 @default.
• W2068391719 cites W1995871176 @default.
• W2068391719 cites W1995994862 @default.
• W2068391719 cites W2014664217 @default.
• W2068391719 cites W2014720377 @default.
• W2068391719 cites W2015942279 @default.
• W2068391719 cites W2054401823 @default.
• W2068391719 cites W2054534751 @default.
• W2068391719 cites W2062530800 @default.
• W2068391719 cites W2067900905 @default.
• W2068391719 cites W2069359746 @default.
• W2068391719 cites W2071888775 @default.
• W2068391719 cites W2076024702 @default.
• W2068391719 cites W2094678638 @default.
• W2068391719 cites W2096262922 @default.
• W2068391719 cites W2126803505 @default.
• W2068391719 cites W2134566626 @default.
• W2068391719 cites W2143678448 @default.
• W2068391719 cites W2155520512 @default.
• W2068391719 cites W2161933333 @default.
• W2068391719 cites W2310575043 @default.
• W2068391719 doi "https://doi.org/10.1007/s10295-004-0181-2" @default.
• W2068391719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15662545" @default.
• W2068391719 hasPublicationYear "2004" @default.
• W2068391719 type Work @default.
• W2068391719 sameAs 2068391719 @default.
• W2068391719 citedByCount "19" @default.
• W2068391719 countsByYear W20683917192012 @default.
• W2068391719 countsByYear W20683917192013 @default.
• W2068391719 countsByYear W20683917192014 @default.
• W2068391719 countsByYear W20683917192016 @default.
• W2068391719 countsByYear W20683917192019 @default.
• W2068391719 countsByYear W20683917192020 @default.
• W2068391719 countsByYear W20683917192021 @default.
• W2068391719 countsByYear W20683917192023 @default.
• W2068391719 crossrefType "journal-article" @default.
• W2068391719 hasAuthorship W2068391719A5066789761 @default.
• W2068391719 hasAuthorship W2068391719A5085070545 @default.
• W2068391719 hasConcept C100544194 @default.
• W2068391719 hasConcept C104317684 @default.
• W2068391719 hasConcept C143065580 @default.
• W2068391719 hasConcept C159641895 @default.
• W2068391719 hasConcept C178790620 @default.
• W2068391719 hasConcept C185592680 @default.
• W2068391719 hasConcept C2777313579 @default.
• W2068391719 hasConcept C2777879272 @default.
• W2068391719 hasConcept C2779548226 @default.
• W2068391719 hasConcept C2780412452 @default.
• W2068391719 hasConcept C2781372317 @default.
• W2068391719 hasConcept C40767141 @default.
• W2068391719 hasConcept C515207424 @default.
• W2068391719 hasConcept C55493867 @default.
• W2068391719 hasConceptScore W2068391719C100544194 @default.
• W2068391719 hasConceptScore W2068391719C104317684 @default.
• W2068391719 hasConceptScore W2068391719C143065580 @default.
• W2068391719 hasConceptScore W2068391719C159641895 @default.
• W2068391719 hasConceptScore W2068391719C178790620 @default.
• W2068391719 hasConceptScore W2068391719C185592680 @default.
• W2068391719 hasConceptScore W2068391719C2777313579 @default.
• W2068391719 hasConceptScore W2068391719C2777879272 @default.
• W2068391719 hasConceptScore W2068391719C2779548226 @default.
• W2068391719 hasConceptScore W2068391719C2780412452 @default.
• W2068391719 hasConceptScore W2068391719C2781372317 @default.
• W2068391719 hasConceptScore W2068391719C40767141 @default.
• W2068391719 hasConceptScore W2068391719C515207424 @default.
• W2068391719 hasConceptScore W2068391719C55493867 @default.
• W2068391719 hasIssue "12" @default.
• W2068391719 hasLocation W20683917191 @default.
• W2068391719 hasLocation W20683917192 @default.
• W2068391719 hasOpenAccess W2068391719 @default.
• W2068391719 hasPrimaryLocation W20683917191 @default.
• W2068391719 hasRelatedWork W1974455305 @default.
• W2068391719 hasRelatedWork W2003707792 @default.
• W2068391719 hasRelatedWork W2013900111 @default.
• W2068391719 hasRelatedWork W2014194130 @default.
• W2068391719 hasRelatedWork W2022615595 @default.
• W2068391719 hasRelatedWork W2023763937 @default.
• W2068391719 hasRelatedWork W2047939538 @default.
• W2068391719 hasRelatedWork W2068391719 @default.
• W2068391719 hasRelatedWork W3193951896 @default.
• W2068391719 hasRelatedWork W4297141779 @default.
• W2068391719 hasVolume "31" @default.
• W2068391719 isParatext "false" @default.

• next