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- W2068408236 abstract "Both common buckwheat ( Fagopyrum esculentum ) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species." @default.
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- W2068408236 date "2002-01-01" @default.
- W2068408236 modified "2023-10-16" @default.
- W2068408236 title "Screening and Selection of Hypoallergenic Buckwheat Species" @default.
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- W2068408236 doi "https://doi.org/10.1100/tsw.2002.157" @default.
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