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• W2068441311 abstract "A new type of sol−gel organic−inorganic hybrid material was developed and used for the fabrication of an amperometric hydrogen peroxide biosensor. This material was prepared from natural chitosan and recently introduced completely water-soluble precursor, tetrakis(2-hydroxyethyl) orthosilicates (THEOS), by the sol−gel process without the addition of organic solvents and catalysts. The gelation time for the sol−gel transition and dynamic rheological properties of the resultant gel matrix could be modulated by the amount of added THEOS. The structure of the hybrid gel was made up of a network and spherical particles, as confirmed by SEM observation. By electrochemical experiments, it was found that such a hybrid gel matrix could retain the native biocatalytic activity of the entrapped horseradish peroxidase and provide a fast amperometric response to hydrogen peroxide. The linear range for the determination of hydrogen peroxide was found to be from 1.0 × 10-6 to 2.5 × 10-4 mol/L with a detection limit of 4.0 × 10-7 mol/L. The apparent Michaelis−Menten constant was determined to be 2.198 mmol/L. To improve the analytical characteristics of the fabricated biosensor, the effects of applied potential and pH value on the steady-state current were studied. Under the optimized experimental conditions, the fabricated biosensor was found to have good analytical performance, reproducibility, and storage stability." @default.
• W2068441311 created "2016-06-24" @default.
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• W2068441311 date "2006-11-16" @default.
• W2068441311 modified "2023-09-27" @default.
• W2068441311 title "Using Novel Polysaccharide−Silica Hybrid Material to Construct An Amperometric Biosensor for Hydrogen Peroxide" @default.
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• W2068441311 doi "https://doi.org/10.1021/jp0657078" @default.
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