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• W2068456078 abstract "A sensitive glutamate biosensor is prepared based on glutamate dehydrogenase/vertically aligned carbon nanotubes (GLDH, VACNTs). Vertically aligned carbon nanotubes were grown on a silicon substrate by direct current plasma enhanced chemical vapor deposition (DC-PECVD) method. The electrochemical behavior of the synthesized VACNTs was investigated by cyclic voltammetry and electrochemical impedance spectroscopic methods. Glutamate dehydrogenase covalently attached on tip of VACNTs. The electrochemical performance of the electrode for detection of glutamate was investigated by cyclic and differential pulse voltammetry. Differential pulse voltammetric determinations of glutamate are performed in mediator-less condition and also, in the presence of 1 and 5 μM thionine as electron mediator. The linear calibration curve of the concentration of glutamate versus peak current is investigated in a wide range of 0.1–500 μM. The mediator-less biosensor has a low detection limit of 57 nM and two linear ranges of 0.1–20 μM with a sensitivity of 0.976 mA mM−1 cm−2 and 20–300 μM with a sensitivity of 0.182 mA mM−1 cm−2. In the presence of 1 μM thionine as an electron mediator, the prepared biosensor shows a low detection limit of 68 nM and two linear ranges of 0.1–20 with a calibration sensitivity of 1.17 mA mM−1 cm−2 and 20–500 μM with a sensitivity of 0.153 mA mM−1 cm−2. The effects of the other biological compounds on the voltammetric behavior of the prepared biosensor and its response stability are investigated. The results are demonstrated that the GLDH/VACNTs electrode even without electron mediator is a suitable basic electrode for detection of glutamate." @default.
• W2068456078 created "2016-06-24" @default.
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• W2068456078 date "2012-01-01" @default.
• W2068456078 modified "2023-09-26" @default.
• W2068456078 title "Mediator-less highly sensitive voltammetric detection of glutamate using glutamate dehydrogenase/vertically aligned CNTs grown on silicon substrate" @default.
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• W2068456078 doi "https://doi.org/10.1016/j.bios.2011.10.002" @default.
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