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- W2068478826 abstract "Abstract Electron microscopic length measurement of Eco RI limit digest products of mature P2 DNA reveals four fragments, A, B, C and D, of defined length. Under our standard conditions, the efficiency of plating of P2 on an RI-carrying strain is 7–10%. An exception is the deletion mutant P2 del2 having a plating efficiency of 30–40%. Electron microscopy of Eco RI digests of P2 del2 DNA shows that del2 removes the middle RI-sensitive site. Since the location of del2 is precisely known from previous work, and the two end segments are sometimes observed joined through their cohesive ends, it is possible to locate the three sites (leftmost, middle, and rightmost) at 10.5, 47.5, and 79.9% from the left end of P2 DNA, respectively. The rightmost site is most probably within cistron B. A mutant, P2 del2 sr1 , was isolated which has a plating efficiency of 70–90% on the RI-carrying host. Electron microscopy of P2 del2 sr1 DNA and phage crosses show that mutation sr1 makes the rightmost site resistant to Eco R1. The sr1 mutation, when separated from del2 , gives a plating efficiency of 20–30% on the RI-carrying host. It would seem that the three sites do not contribute equally to the lowering of the efficiency of plating." @default.
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- W2068478826 date "1977-09-01" @default.
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- W2068478826 title "Restriction of bacteriophage P2 by the Escherichia coli RI plasmid, and in vitro cleavage of its DNA by the EcoRI endonuclease" @default.
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- W2068478826 doi "https://doi.org/10.1016/0042-6822(77)90161-1" @default.
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