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- W2068507812 abstract "INTRODUCTION This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trace amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water." @default.
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- W2068507812 date "2009-12-01" @default.
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- W2068507812 title "Isolation of Sea Squirt <i>(Ciona)</i> Gametes, Fertilization, Dechorionation, and Development: Figure 1." @default.
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