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• W2068644302 abstract "In this study, a rapid quantitative method using TaqMan-based real-time reverse transcription-polymerase chain reaction (qPCR-RT) has been developed for estimating the titers of measles, mumps and rubella (MMR) viruses in infected cell culture supernatants. The qPCR-RT assay was demonstrated to be a specific, sensitive, efficient and reproducible method. For MMR viral samples obtained during MMR viral propagations in Vero cells at a different multiplicity of infection, titers determined by the qPCR-RT assay have been compared with estimates of infectious virus obtained by a traditional commonly used method for MMR viruses – 50% cell culture infective dose (CCID50) assay, in paired samples. Pearson analysis evidenced a significant correlation between both methods for a certain period after viral inoculation. Furthermore, the established qPCR-RT assay was faster and less-laborious. The developed method could be used as an alternative method or a supplementary tool for the routine titer estimation during MMR vaccine production." @default.
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• W2068644302 date "2013-01-01" @default.
• W2068644302 modified "2023-09-27" @default.
• W2068644302 title "Quantification of measles, mumps and rubella viruses using real-time quantitative TaqMan-based RT-PCR assay" @default.
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• W2068644302 doi "https://doi.org/10.1016/j.jviromet.2012.09.011" @default.
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