Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068668897> ?p ?o ?g. }
- W2068668897 endingPage "115" @default.
- W2068668897 startingPage "109" @default.
- W2068668897 abstract "Intelligent gene delivery systems based on physiologically triggered reversible shielding technology have evinced enormous interest due to their potential in vivo applications. In the present work, an acid-labile block copolymer consisting of poly(ethylene glycol) and poly(2-(dimethylamino)ethyl methacrylate) segments connected through a cyclic ortho ester linkage (PEG-a-PDMAEMA) was synthesized by atom transfer radical polymerization of DMAEMA using a PEG macroinitiator with an acid-cleavable end group. PEG-a-PDMAEMA condensed with plasmid DNA formed polyplex nanoparticles with an acid-triggered reversible PEG shield. The pH-dependent shielding/deshielding effect of PEG chains on the polyplex particles were evaluated by ζ potential and size measurements. At pH 7.4, polyplexes generated from PEG-a-PDMAEMA exhibited smaller particle size, lower surface charge, reduced interaction with erythrocytes, and less cytotoxicity compared to PDMAEMA-derived polyplexes. At pH 5.0, ζ potential of polyplexes formed from PEG-a-PDMAEMA increased, leveled up after 2 h of incubation and gradual aggregation occurred in the presence of bovine serum albumin (BSA). In contrast, the stably shielded polyplexes formed by DNA and an acid-stable block copolymer, PEG-b-PDMAEMA, did not change in size and ζ potential in 6 h. In vitro transfection efficiency of the acid-labile copolymer greatly increased after 6 h incubation at pH 5.0, approaching the same level of PDMAEMA, whereas there was only slight increase in efficiency for the stable copolymer, PEG-b-PDMAEMA." @default.
- W2068668897 created "2016-06-24" @default.
- W2068668897 creator A5003267672 @default.
- W2068668897 creator A5005864617 @default.
- W2068668897 creator A5031795955 @default.
- W2068668897 creator A5071249790 @default.
- W2068668897 creator A5072684728 @default.
- W2068668897 creator A5082944340 @default.
- W2068668897 creator A5090162223 @default.
- W2068668897 date "2007-12-19" @default.
- W2068668897 modified "2023-10-14" @default.
- W2068668897 title "An Acid-Labile Block Copolymer of PDMAEMA and PEG as Potential Carrier for Intelligent Gene Delivery Systems" @default.
- W2068668897 cites W1560355462 @default.
- W2068668897 cites W1602020336 @default.
- W2068668897 cites W1966390198 @default.
- W2068668897 cites W1967163411 @default.
- W2068668897 cites W1972128873 @default.
- W2068668897 cites W1972804296 @default.
- W2068668897 cites W1973174294 @default.
- W2068668897 cites W1973458116 @default.
- W2068668897 cites W1976811878 @default.
- W2068668897 cites W1977721883 @default.
- W2068668897 cites W1979099550 @default.
- W2068668897 cites W1989868163 @default.
- W2068668897 cites W1992998779 @default.
- W2068668897 cites W2000200774 @default.
- W2068668897 cites W2000582064 @default.
- W2068668897 cites W2003414091 @default.
- W2068668897 cites W2006921898 @default.
- W2068668897 cites W2007885143 @default.
- W2068668897 cites W2010228315 @default.
- W2068668897 cites W2012057893 @default.
- W2068668897 cites W2012907151 @default.
- W2068668897 cites W2017720816 @default.
- W2068668897 cites W2020246410 @default.
- W2068668897 cites W2024719999 @default.
- W2068668897 cites W2024907303 @default.
- W2068668897 cites W2026085888 @default.
- W2068668897 cites W2031185644 @default.
- W2068668897 cites W2031632278 @default.
- W2068668897 cites W2033029089 @default.
- W2068668897 cites W2039208932 @default.
- W2068668897 cites W2044524539 @default.
- W2068668897 cites W2048456201 @default.
- W2068668897 cites W2049261652 @default.
- W2068668897 cites W2050932091 @default.
- W2068668897 cites W2055020116 @default.
- W2068668897 cites W2057576065 @default.
- W2068668897 cites W2057848113 @default.
- W2068668897 cites W2074870430 @default.
- W2068668897 cites W2077850392 @default.
- W2068668897 cites W2078447684 @default.
- W2068668897 cites W2078534751 @default.
- W2068668897 cites W2080071319 @default.
- W2068668897 cites W2083033801 @default.
- W2068668897 cites W2083609039 @default.
- W2068668897 cites W2087004282 @default.
- W2068668897 cites W2093104420 @default.
- W2068668897 cites W2093971940 @default.
- W2068668897 cites W2094101506 @default.
- W2068668897 cites W2133276513 @default.
- W2068668897 cites W2133923986 @default.
- W2068668897 cites W2143975668 @default.
- W2068668897 cites W2321451097 @default.
- W2068668897 cites W2949091778 @default.
- W2068668897 cites W2950656567 @default.
- W2068668897 cites W4207061948 @default.
- W2068668897 cites W91990295 @default.
- W2068668897 doi "https://doi.org/10.1021/bm7008747" @default.
- W2068668897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18088093" @default.
- W2068668897 hasPublicationYear "2007" @default.
- W2068668897 type Work @default.
- W2068668897 sameAs 2068668897 @default.
- W2068668897 citedByCount "207" @default.
- W2068668897 countsByYear W20686688972012 @default.
- W2068668897 countsByYear W20686688972013 @default.
- W2068668897 countsByYear W20686688972014 @default.
- W2068668897 countsByYear W20686688972015 @default.
- W2068668897 countsByYear W20686688972016 @default.
- W2068668897 countsByYear W20686688972017 @default.
- W2068668897 countsByYear W20686688972018 @default.
- W2068668897 countsByYear W20686688972019 @default.
- W2068668897 countsByYear W20686688972020 @default.
- W2068668897 countsByYear W20686688972021 @default.
- W2068668897 countsByYear W20686688972022 @default.
- W2068668897 countsByYear W20686688972023 @default.
- W2068668897 crossrefType "journal-article" @default.
- W2068668897 hasAuthorship W2068668897A5003267672 @default.
- W2068668897 hasAuthorship W2068668897A5005864617 @default.
- W2068668897 hasAuthorship W2068668897A5031795955 @default.
- W2068668897 hasAuthorship W2068668897A5071249790 @default.
- W2068668897 hasAuthorship W2068668897A5072684728 @default.
- W2068668897 hasAuthorship W2068668897A5082944340 @default.
- W2068668897 hasAuthorship W2068668897A5090162223 @default.
- W2068668897 hasConcept C10138342 @default.
- W2068668897 hasConcept C104317684 @default.
- W2068668897 hasConcept C12554922 @default.
- W2068668897 hasConcept C132050475 @default.