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- W2068683693 abstract "Spermine-graft-dextran (Spe-g-Dex) copolymer was synthesized and used as a non-covalent coating for the separation of proteins and neurotransmitters by capillary electrophoresis. The coating was obtained via flushing the capillary with 1.0% Spe-g-Dex copolymer solution for 2 min. Electroosmotic flow (EOF) was strongly suppressed, ranging from −1.60 × 10 −9 to 3.65 × 10 −9 m 2 V −1 s −1 . Effect of experimental conditions, such as the copolymer concentration, the concentration and pH of the background electrolyte (BGE), on the Spe-g-Dex coating was investigated. Separation of lysozyme, cytochrome c, ribonuclease A and α-chymotrypsinogen yielded high separation efficiencies ranging from 141 000 to 303 000 plates/m and recoveries from 85.4% to 98.3% at pH 4.0 (284.0 mM sodium acetate–acetic acid buffer, I = 50 mM). Run-to-run repeatabilities and day-to-day, and capillary-to-capillary reproducibilities were all below 1.7%. In addition, Spe-g-Dex coating allowed the successful separation of five neurotransmitters, 5-hydroxytryptamine, dopamine, epinephrine, isoprenaline, dobuamine at pH 4.0 with high separation efficiencies of 290 000–449 000 plates/m." @default.
- W2068683693 created "2016-06-24" @default.
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- W2068683693 date "2010-07-01" @default.
- W2068683693 modified "2023-10-18" @default.
- W2068683693 title "Spermine-graft-dextran non-covalent copolymer as coating material in separation of basic proteins and neurotransmitters by capillary electrophoresis" @default.
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- W2068683693 doi "https://doi.org/10.1016/j.chroma.2010.06.019" @default.
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