FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068707554> ?p ?o ?g. }

• W2068707554 endingPage "17" @default.
• W2068707554 startingPage "12" @default.
• W2068707554 abstract "To determine whether autoclave sterilization eradicates human papillomavirus (HPV) DNA on specula and instruments used to treat women with cervical neoplasia.Specula and instruments used in two referral colposcopy clinics were evaluated to determine the PGMY9/11 primer system's ability to amplify residual HPV DNA. Each speculum and instrument was sampled with a Dacron swab and stored in PreservCyt solution (Cytyc Corporation, Marlborough, MA) at 4 degrees C. DNA amplification was performed under standard conditions with appropriate controls followed by HPV typing using the reverse line blot test (Roche Molecular Systems, Alameda, CA). Once validated, the same polymerase chain reaction method was used on autoclave-sterilized specula and biopsy instruments and heated glass bead- and Cidex bath (Johnson & Johnson, New Brunswick, NJ)-sterilized instruments. All results, with appropriate positive and negative controls, were confirmed in triplicate.A total of 140 instruments (70 used and 70 autoclaved) were sampled for residual HPV DNA. Five samples in the contaminated specula arm were excluded from analysis secondary to insufficient sampling. Of the remaining samples, 52.3% (34/65) of contaminated instruments-both specula and biopsy instruments-had detectable HPV DNA. Fifty-five percent of contaminated biopsy instruments (11/20) were positive and 51.1% of contaminated specula (23/45) were positive. All 70 autoclaved samples (50 specula and 20 biopsy instruments) were negative for residual HPV DNA or beta-globin. One instrument in the glass bead and Cidex group that was presumed sterile was positive for HPV 16 DNA.The PGMY9/11 primer system is an effective method to detect residual HPV DNA. Autoclave sterilization appears to eradicate HPV DNA to levels undetectable with this sensitive assay, whereas heated glass beads followed by Cidex bath appears to be inadequate methods. These results suggest that autoclave sterilization is effective when using nondisposable instruments and should be the method of choice in studies using polymerase chain reaction-based amplification of HPV DNA." @default.
• W2068707554 created "2016-06-24" @default.
• W2068707554 creator A5049013335 @default.
• W2068707554 creator A5065108808 @default.
• W2068707554 creator A5076400654 @default.
• W2068707554 date "2007-01-01" @default.
• W2068707554 modified "2023-09-27" @default.
• W2068707554 title "Autoclave Sterilization of Instruments Used on Women with Cervical Neoplasia Is an Effective Method of Eradicating Residual Human Papillomavirus DNA" @default.
• W2068707554 cites W1812361043 @default.
• W2068707554 cites W2076086251 @default.
• W2068707554 cites W2087162682 @default.
• W2068707554 cites W2090751944 @default.
• W2068707554 cites W2095688730 @default.
• W2068707554 cites W2096478290 @default.
• W2068707554 cites W2121189112 @default.
• W2068707554 cites W2131330171 @default.
• W2068707554 cites W2151164686 @default.
• W2068707554 cites W2151683569 @default.
• W2068707554 doi "https://doi.org/10.1097/01.lgt.0000229565.19364.cc" @default.
• W2068707554 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17194945" @default.
• W2068707554 hasPublicationYear "2007" @default.
• W2068707554 type Work @default.
• W2068707554 sameAs 2068707554 @default.
• W2068707554 citedByCount "7" @default.
• W2068707554 countsByYear W20687075542014 @default.
• W2068707554 countsByYear W20687075542015 @default.
• W2068707554 countsByYear W20687075542018 @default.
• W2068707554 countsByYear W20687075542020 @default.
• W2068707554 crossrefType "journal-article" @default.
• W2068707554 hasAuthorship W2068707554A5049013335 @default.
• W2068707554 hasAuthorship W2068707554A5065108808 @default.
• W2068707554 hasAuthorship W2068707554A5076400654 @default.
• W2068707554 hasConcept C121608353 @default.
• W2068707554 hasConcept C126322002 @default.
• W2068707554 hasConcept C148761745 @default.
• W2068707554 hasConcept C162324750 @default.
• W2068707554 hasConcept C2775934546 @default.
• W2068707554 hasConcept C2776117191 @default.
• W2068707554 hasConcept C2778220009 @default.
• W2068707554 hasConcept C2983132609 @default.
• W2068707554 hasConcept C536366893 @default.
• W2068707554 hasConcept C556758197 @default.
• W2068707554 hasConcept C71924100 @default.
• W2068707554 hasConceptScore W2068707554C121608353 @default.
• W2068707554 hasConceptScore W2068707554C126322002 @default.
• W2068707554 hasConceptScore W2068707554C148761745 @default.
• W2068707554 hasConceptScore W2068707554C162324750 @default.
• W2068707554 hasConceptScore W2068707554C2775934546 @default.
• W2068707554 hasConceptScore W2068707554C2776117191 @default.
• W2068707554 hasConceptScore W2068707554C2778220009 @default.
• W2068707554 hasConceptScore W2068707554C2983132609 @default.
• W2068707554 hasConceptScore W2068707554C536366893 @default.
• W2068707554 hasConceptScore W2068707554C556758197 @default.
• W2068707554 hasConceptScore W2068707554C71924100 @default.
• W2068707554 hasIssue "1" @default.
• W2068707554 hasLocation W20687075541 @default.
• W2068707554 hasLocation W20687075542 @default.
• W2068707554 hasOpenAccess W2068707554 @default.
• W2068707554 hasPrimaryLocation W20687075541 @default.
• W2068707554 hasRelatedWork W1486082332 @default.
• W2068707554 hasRelatedWork W2008674534 @default.
• W2068707554 hasRelatedWork W2009885920 @default.
• W2068707554 hasRelatedWork W2148481825 @default.
• W2068707554 hasRelatedWork W2351658163 @default.
• W2068707554 hasRelatedWork W2353295690 @default.
• W2068707554 hasRelatedWork W2356437938 @default.
• W2068707554 hasRelatedWork W2497334881 @default.
• W2068707554 hasRelatedWork W2760612246 @default.
• W2068707554 hasRelatedWork W3032588439 @default.
• W2068707554 hasVolume "11" @default.
• W2068707554 isParatext "false" @default.
• W2068707554 isRetracted "false" @default.
• W2068707554 magId "2068707554" @default.
• W2068707554 workType "article" @default.