Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068722709> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2068722709 endingPage "641" @default.
- W2068722709 startingPage "637" @default.
- W2068722709 abstract "The NADH and NAD+ dependent reactions catalyzed by glutamate dehydrogenase (GDH) from sterile cultures of Lemna minor are completely inactivated by EDTA. The activities of both reactions can be fully restored by addition of Ca2+ and to a lesser extent Mn2+, Zn2+, Sr2+ or La3+, whereas Mg2+ reactivates only the NAD+ dependent reaction. Activation of the NADH reaction by Ca2+ has been studied by using partially purified, EDTA pretreated, and Mg2+ saturated GDH preparations. Saturation kinetic curves with Ca2+ were always sigmoidal, whereas saturation plots for the 3 substrates of the aminating reaction at various fixed Ca2+ concentrations showed normal Michaelis kinetics. However, a pronounced substrate inhibition at low Ca2+ levels was found, particularly with NH4+ and NADH. Product inhibition studies revealed unchanged enzyme substrate binding characteristics for NADH and 2-oxoglutarate in the Ca2+ free enzyme. A drastic alteration was established for the third substrate NH4+. The kinetic data suggest that Ca2+ governs an equilibrium between a catalytically inactive (Ca2+ free) and an active (Ca2+ saturated) enzyme form. Inactivation by removal of Ca2+ is related to an alteration in the binding characteristics or binding sequence of the substrate NH4+." @default.
- W2068722709 created "2016-06-24" @default.
- W2068722709 creator A5009977783 @default.
- W2068722709 creator A5055791745 @default.
- W2068722709 date "1978-01-01" @default.
- W2068722709 modified "2023-10-17" @default.
- W2068722709 title "Control of glutamate dehydrogenase from Lemna minor by divalent metal ions" @default.
- W2068722709 cites W1964821798 @default.
- W2068722709 cites W1971036277 @default.
- W2068722709 cites W1993636839 @default.
- W2068722709 cites W2001186590 @default.
- W2068722709 cites W2039405183 @default.
- W2068722709 cites W2077824139 @default.
- W2068722709 cites W2130637304 @default.
- W2068722709 cites W4253172274 @default.
- W2068722709 doi "https://doi.org/10.1016/s0031-9422(00)94199-2" @default.
- W2068722709 hasPublicationYear "1978" @default.
- W2068722709 type Work @default.
- W2068722709 sameAs 2068722709 @default.
- W2068722709 citedByCount "12" @default.
- W2068722709 crossrefType "journal-article" @default.
- W2068722709 hasAuthorship W2068722709A5009977783 @default.
- W2068722709 hasAuthorship W2068722709A5055791745 @default.
- W2068722709 hasConcept C121332964 @default.
- W2068722709 hasConcept C132000320 @default.
- W2068722709 hasConcept C148898269 @default.
- W2068722709 hasConcept C170493617 @default.
- W2068722709 hasConcept C178790620 @default.
- W2068722709 hasConcept C181199279 @default.
- W2068722709 hasConcept C185592680 @default.
- W2068722709 hasConcept C18903297 @default.
- W2068722709 hasConcept C2776317432 @default.
- W2068722709 hasConcept C2777202666 @default.
- W2068722709 hasConcept C2777289219 @default.
- W2068722709 hasConcept C28718788 @default.
- W2068722709 hasConcept C2994074225 @default.
- W2068722709 hasConcept C34771814 @default.
- W2068722709 hasConcept C41183919 @default.
- W2068722709 hasConcept C55493867 @default.
- W2068722709 hasConcept C56856141 @default.
- W2068722709 hasConcept C61174792 @default.
- W2068722709 hasConcept C62520636 @default.
- W2068722709 hasConcept C71240020 @default.
- W2068722709 hasConcept C74884574 @default.
- W2068722709 hasConcept C75520062 @default.
- W2068722709 hasConcept C86803240 @default.
- W2068722709 hasConceptScore W2068722709C121332964 @default.
- W2068722709 hasConceptScore W2068722709C132000320 @default.
- W2068722709 hasConceptScore W2068722709C148898269 @default.
- W2068722709 hasConceptScore W2068722709C170493617 @default.
- W2068722709 hasConceptScore W2068722709C178790620 @default.
- W2068722709 hasConceptScore W2068722709C181199279 @default.
- W2068722709 hasConceptScore W2068722709C185592680 @default.
- W2068722709 hasConceptScore W2068722709C18903297 @default.
- W2068722709 hasConceptScore W2068722709C2776317432 @default.
- W2068722709 hasConceptScore W2068722709C2777202666 @default.
- W2068722709 hasConceptScore W2068722709C2777289219 @default.
- W2068722709 hasConceptScore W2068722709C28718788 @default.
- W2068722709 hasConceptScore W2068722709C2994074225 @default.
- W2068722709 hasConceptScore W2068722709C34771814 @default.
- W2068722709 hasConceptScore W2068722709C41183919 @default.
- W2068722709 hasConceptScore W2068722709C55493867 @default.
- W2068722709 hasConceptScore W2068722709C56856141 @default.
- W2068722709 hasConceptScore W2068722709C61174792 @default.
- W2068722709 hasConceptScore W2068722709C62520636 @default.
- W2068722709 hasConceptScore W2068722709C71240020 @default.
- W2068722709 hasConceptScore W2068722709C74884574 @default.
- W2068722709 hasConceptScore W2068722709C75520062 @default.
- W2068722709 hasConceptScore W2068722709C86803240 @default.
- W2068722709 hasIssue "4" @default.
- W2068722709 hasLocation W20687227091 @default.
- W2068722709 hasOpenAccess W2068722709 @default.
- W2068722709 hasPrimaryLocation W20687227091 @default.
- W2068722709 hasRelatedWork W1509807418 @default.
- W2068722709 hasRelatedWork W1863070284 @default.
- W2068722709 hasRelatedWork W2006839728 @default.
- W2068722709 hasRelatedWork W2025788670 @default.
- W2068722709 hasRelatedWork W2029587448 @default.
- W2068722709 hasRelatedWork W2033150961 @default.
- W2068722709 hasRelatedWork W2068722709 @default.
- W2068722709 hasRelatedWork W2070563424 @default.
- W2068722709 hasRelatedWork W2210033408 @default.
- W2068722709 hasRelatedWork W2494851022 @default.
- W2068722709 hasVolume "17" @default.
- W2068722709 isParatext "false" @default.
- W2068722709 isRetracted "false" @default.
- W2068722709 magId "2068722709" @default.
- W2068722709 workType "article" @default.