Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068742012> ?p ?o ?g. }
- W2068742012 endingPage "e88409" @default.
- W2068742012 startingPage "e88409" @default.
- W2068742012 abstract "Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the proline knot motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants." @default.
- W2068742012 created "2016-06-24" @default.
- W2068742012 creator A5021541475 @default.
- W2068742012 creator A5048477459 @default.
- W2068742012 creator A5054595017 @default.
- W2068742012 creator A5065528836 @default.
- W2068742012 creator A5087799528 @default.
- W2068742012 date "2014-02-06" @default.
- W2068742012 modified "2023-09-26" @default.
- W2068742012 title "Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)" @default.
- W2068742012 cites W1965654635 @default.
- W2068742012 cites W1966713342 @default.
- W2068742012 cites W1967900452 @default.
- W2068742012 cites W1968633108 @default.
- W2068742012 cites W1972771722 @default.
- W2068742012 cites W1981819223 @default.
- W2068742012 cites W1986080201 @default.
- W2068742012 cites W1990966549 @default.
- W2068742012 cites W1994145712 @default.
- W2068742012 cites W1997938589 @default.
- W2068742012 cites W1998812021 @default.
- W2068742012 cites W2002631115 @default.
- W2068742012 cites W2002736489 @default.
- W2068742012 cites W2003935644 @default.
- W2068742012 cites W2008801911 @default.
- W2068742012 cites W2010808514 @default.
- W2068742012 cites W2013930644 @default.
- W2068742012 cites W2014969235 @default.
- W2068742012 cites W2015111200 @default.
- W2068742012 cites W2023919356 @default.
- W2068742012 cites W2025175744 @default.
- W2068742012 cites W2027961934 @default.
- W2068742012 cites W2029932852 @default.
- W2068742012 cites W2035869261 @default.
- W2068742012 cites W2039349831 @default.
- W2068742012 cites W2048341740 @default.
- W2068742012 cites W2053200244 @default.
- W2068742012 cites W2054352354 @default.
- W2068742012 cites W2055043387 @default.
- W2068742012 cites W2056239986 @default.
- W2068742012 cites W2058527493 @default.
- W2068742012 cites W2060531604 @default.
- W2068742012 cites W2063297728 @default.
- W2068742012 cites W2064606101 @default.
- W2068742012 cites W2065002160 @default.
- W2068742012 cites W2065509325 @default.
- W2068742012 cites W2067335713 @default.
- W2068742012 cites W2070360918 @default.
- W2068742012 cites W2074196067 @default.
- W2068742012 cites W2075117070 @default.
- W2068742012 cites W2081906422 @default.
- W2068742012 cites W2087046839 @default.
- W2068742012 cites W2087314450 @default.
- W2068742012 cites W2087967383 @default.
- W2068742012 cites W2092157454 @default.
- W2068742012 cites W2103973399 @default.
- W2068742012 cites W2105035326 @default.
- W2068742012 cites W2106882534 @default.
- W2068742012 cites W2107277218 @default.
- W2068742012 cites W2107845684 @default.
- W2068742012 cites W2108467818 @default.
- W2068742012 cites W2109792995 @default.
- W2068742012 cites W2112345800 @default.
- W2068742012 cites W2112965894 @default.
- W2068742012 cites W2114661018 @default.
- W2068742012 cites W2120381562 @default.
- W2068742012 cites W2121393621 @default.
- W2068742012 cites W2125346198 @default.
- W2068742012 cites W2127752221 @default.
- W2068742012 cites W2130926483 @default.
- W2068742012 cites W2134586609 @default.
- W2068742012 cites W2135836594 @default.
- W2068742012 cites W2137015675 @default.
- W2068742012 cites W2141391392 @default.
- W2068742012 cites W2146756331 @default.
- W2068742012 cites W2148968778 @default.
- W2068742012 cites W2149836365 @default.
- W2068742012 cites W2151986753 @default.
- W2068742012 cites W2157220396 @default.
- W2068742012 cites W2161910309 @default.
- W2068742012 cites W2163357966 @default.
- W2068742012 cites W2168560917 @default.
- W2068742012 cites W2465323533 @default.
- W2068742012 cites W2915160214 @default.
- W2068742012 cites W2915880292 @default.
- W2068742012 cites W2918031289 @default.
- W2068742012 doi "https://doi.org/10.1371/journal.pone.0088409" @default.
- W2068742012 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3916434" @default.
- W2068742012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24516650" @default.
- W2068742012 hasPublicationYear "2014" @default.
- W2068742012 type Work @default.
- W2068742012 sameAs 2068742012 @default.
- W2068742012 citedByCount "16" @default.
- W2068742012 countsByYear W20687420122014 @default.
- W2068742012 countsByYear W20687420122015 @default.
- W2068742012 countsByYear W20687420122016 @default.
- W2068742012 countsByYear W20687420122017 @default.
- W2068742012 countsByYear W20687420122019 @default.