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• W2068753536 abstract "Phage Φ29 protein p4 activates the late A3 promoter and represses the early A2c promoter, in both cases by binding upstream from RNA polymerase (RNAP) and interacting with the C-terminal domain of the RNAP α subunit. To investigate how this interaction leads to activation at P A3 and to repression at P A2c, mutant promoters were constructed. We show that the position of protein p4 relative to that of RNAP, which is different at each promoter, does not dictate the outcome of the interaction. Rather, in the absence of a −35 consensus box for σA-RNAP activation was observed, while in its presence repression occurred. The results support the view that stabilization of RNAP at the promoter over a threshold level leads to repression." @default.
• W2068753536 created "2016-06-24" @default.
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• W2068753536 date "1997-12-01" @default.
• W2068753536 modified "2023-10-18" @default.
• W2068753536 title "Transcription Activation or Repression by Phage Φ29 Protein p4 Depends on the Strength of the RNA Polymerase–Promoter Interactions" @default.
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• W2068753536 doi "https://doi.org/10.1016/s1097-2765(00)80011-8" @default.
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