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- W2068754531 abstract "A cDNA for human interleukin-5 (hIL-5) was created from the hIL-5 gene using site-directed mutagenesis to splice out the introns in vitro. This cDNA was expressed in yeast and baculovirus systems, utilizing in both cases an in-frame fusion to the pre sequence of the α-mating-type factor to direct secretion. The highest level of production was achieved from Sf9 cells using a baculovirus vector in serum-containing medium (2.7 mg/l), whereas in serum-free medium ten times less hIL-5 was produced. In the yeast system much lower levels of hIL-5 were produced (12.5 μg/l). Recombinant hIL-5 was purified to homogeneity from serum-free baculovirus cultures. The rhIL-5 consisted of a 30-kDa homodimer linked by disulfide bridging. The purified recombinant protein had a specific activity on murine BCL1 cells of 1.5x104 U/mg, of 3x105 U/mg in the murine eosinophil differentiation factor assay, and 2.4x107 U/mg in a human peripheral eosinophil maintenance assay." @default.
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- W2068754531 date "1991-03-01" @default.
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- W2068754531 title "Production and purification of recombinant human interleukin-5 from yeast and baculovirus expression systems" @default.
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- W2068754531 doi "https://doi.org/10.1111/j.1432-1033.1991.tb15858.x" @default.
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