Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068756771> ?p ?o ?g. }
- W2068756771 endingPage "5977" @default.
- W2068756771 startingPage "5967" @default.
- W2068756771 abstract "The processing of DNA double-strand breaks (DSBs) into 3' single-stranded tails is the first step of homology-dependent DSB repair. A key player in this process is the highly conserved eukaryotic exonuclease 1 (EXO1), yet its precise mechanism of action has not been rigorously determined. To address this issue, we reconstituted 5'-strand resection in cytosol derived from unfertilized interphase eggs of the frog Xenopus laevis. Xenopus EXO1 (xEXO1) was found to display strong 5'→3' dsDNA exonuclease activity but no significant ssDNA exonuclease activity. Depletion of xEXO1 caused significant inhibition of 5' strand resection. Co-depletion of xEXO1 and Xenopus DNA2 (xDNA2) showed that these two nucleases act in parallel pathways and by distinct mechanisms. While xDNA2 acts on ssDNA unwound mainly by the Xenopus Werner syndrome protein (xWRN), xEXO1 acts directly on dsDNA. Furthermore, xEXO1 and xWRN are required for both the initiation stage and the extension stage of resection. These results reveal important novel information on the mechanism of 5'-strand resection in eukaryotes." @default.
- W2068756771 created "2016-06-24" @default.
- W2068756771 creator A5009985634 @default.
- W2068756771 creator A5034374261 @default.
- W2068756771 creator A5042550088 @default.
- W2068756771 date "2011-04-13" @default.
- W2068756771 modified "2023-09-27" @default.
- W2068756771 title "Mechanistic analysis of Xenopus EXO1's function in 5'-strand resection at DNA double-strand breaks" @default.
- W2068756771 cites W1420696562 @default.
- W2068756771 cites W1501269279 @default.
- W2068756771 cites W1523619671 @default.
- W2068756771 cites W1592309404 @default.
- W2068756771 cites W1958566893 @default.
- W2068756771 cites W1979385111 @default.
- W2068756771 cites W1993026474 @default.
- W2068756771 cites W1997407651 @default.
- W2068756771 cites W2013456924 @default.
- W2068756771 cites W2019239618 @default.
- W2068756771 cites W2021870890 @default.
- W2068756771 cites W2028138760 @default.
- W2068756771 cites W2046535007 @default.
- W2068756771 cites W2052401964 @default.
- W2068756771 cites W2052547134 @default.
- W2068756771 cites W2052881693 @default.
- W2068756771 cites W2053299308 @default.
- W2068756771 cites W2054370981 @default.
- W2068756771 cites W2054914405 @default.
- W2068756771 cites W2062284662 @default.
- W2068756771 cites W2081383594 @default.
- W2068756771 cites W2082981258 @default.
- W2068756771 cites W2094020226 @default.
- W2068756771 cites W2101100530 @default.
- W2068756771 cites W2102107922 @default.
- W2068756771 cites W2115595801 @default.
- W2068756771 cites W2117602431 @default.
- W2068756771 cites W2121781767 @default.
- W2068756771 cites W2124037827 @default.
- W2068756771 cites W2133231925 @default.
- W2068756771 cites W2137428107 @default.
- W2068756771 cites W2143842681 @default.
- W2068756771 cites W2151221696 @default.
- W2068756771 cites W2159431285 @default.
- W2068756771 cites W4249309674 @default.
- W2068756771 doi "https://doi.org/10.1093/nar/gkr216" @default.
- W2068756771 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3152354" @default.
- W2068756771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21490081" @default.
- W2068756771 hasPublicationYear "2011" @default.
- W2068756771 type Work @default.
- W2068756771 sameAs 2068756771 @default.
- W2068756771 citedByCount "26" @default.
- W2068756771 countsByYear W20687567712012 @default.
- W2068756771 countsByYear W20687567712013 @default.
- W2068756771 countsByYear W20687567712014 @default.
- W2068756771 countsByYear W20687567712015 @default.
- W2068756771 countsByYear W20687567712016 @default.
- W2068756771 countsByYear W20687567712017 @default.
- W2068756771 countsByYear W20687567712018 @default.
- W2068756771 countsByYear W20687567712019 @default.
- W2068756771 countsByYear W20687567712020 @default.
- W2068756771 countsByYear W20687567712021 @default.
- W2068756771 crossrefType "journal-article" @default.
- W2068756771 hasAuthorship W2068756771A5009985634 @default.
- W2068756771 hasAuthorship W2068756771A5034374261 @default.
- W2068756771 hasAuthorship W2068756771A5042550088 @default.
- W2068756771 hasBestOaLocation W20687567711 @default.
- W2068756771 hasConcept C104317684 @default.
- W2068756771 hasConcept C153911025 @default.
- W2068756771 hasConcept C2756471 @default.
- W2068756771 hasConcept C2775984157 @default.
- W2068756771 hasConcept C2777501222 @default.
- W2068756771 hasConcept C2779535977 @default.
- W2068756771 hasConcept C54355233 @default.
- W2068756771 hasConcept C552990157 @default.
- W2068756771 hasConcept C86339819 @default.
- W2068756771 hasConcept C86803240 @default.
- W2068756771 hasConcept C94966510 @default.
- W2068756771 hasConcept C95444343 @default.
- W2068756771 hasConceptScore W2068756771C104317684 @default.
- W2068756771 hasConceptScore W2068756771C153911025 @default.
- W2068756771 hasConceptScore W2068756771C2756471 @default.
- W2068756771 hasConceptScore W2068756771C2775984157 @default.
- W2068756771 hasConceptScore W2068756771C2777501222 @default.
- W2068756771 hasConceptScore W2068756771C2779535977 @default.
- W2068756771 hasConceptScore W2068756771C54355233 @default.
- W2068756771 hasConceptScore W2068756771C552990157 @default.
- W2068756771 hasConceptScore W2068756771C86339819 @default.
- W2068756771 hasConceptScore W2068756771C86803240 @default.
- W2068756771 hasConceptScore W2068756771C94966510 @default.
- W2068756771 hasConceptScore W2068756771C95444343 @default.
- W2068756771 hasIssue "14" @default.
- W2068756771 hasLocation W20687567711 @default.
- W2068756771 hasLocation W20687567712 @default.
- W2068756771 hasLocation W20687567713 @default.
- W2068756771 hasLocation W20687567714 @default.
- W2068756771 hasOpenAccess W2068756771 @default.
- W2068756771 hasPrimaryLocation W20687567711 @default.
- W2068756771 hasRelatedWork W1044284101 @default.
- W2068756771 hasRelatedWork W1537768102 @default.