FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068767522> ?p ?o ?g. }

• W2068767522 abstract "Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In affected individuals, pyrimidine nucleotides as well as their precursor polymers and their off-path metabolites accumulate in erythrocytes, interfering with their proper function in ways that are not yet fully understood. This report describes the first X-ray structure of a catalytically inactivated variant of murine cN-III with a natural substrate, uridine 5'-monophosphate, in the active site at 1.74Å resolution. The structure captures in an atomic detail the closed conformation that cN-III adopts upon substrate binding. Structure and sequence analysis coupled with enzymatic characterization of several mutants confirmed that the aromatic ring of a nitrogenous base of substrate nucleotide is stabilized by parallel π-stacking interactions with conserved aromatic rings of Trp113 and His68. The nitrogenous base is further stabilized by T-shaped stacking with the conserved aromatic ring of Tyr114, as well as by polar contacts with side chains of Thr66 and Ser117. Two water molecules help to stabilize the nucleotide binding by bridging it to protein residues Asp72 and His68 via hydrogen bonds. Finally, fully conserved Glu96 is responsible for recognition of ribose ring via two hydrogen bonds. The presented substrate complex structure elucidates how cN-III achieves specificity for pyrimidine 5'-nucleotides and how it selects against purine 5'-nucleotides." @default.
• W2068767522 created "2016-06-24" @default.
• W2068767522 creator A5024598524 @default.
• W2068767522 creator A5025942956 @default.
• W2068767522 creator A5031761034 @default.
• W2068767522 creator A5054698608 @default.
• W2068767522 creator A5055625466 @default.
• W2068767522 creator A5062471072 @default.
• W2068767522 creator A5078498918 @default.
• W2068767522 date "2012-11-01" @default.
• W2068767522 modified "2023-09-24" @default.
• W2068767522 title "Structural Basis of Substrate Specificity and Selectivity of Murine Cytosolic 5′-Nucleotidase III" @default.
• W2068767522 cites W1536886974 @default.
• W2068767522 cites W1539796472 @default.
• W2068767522 cites W1841647509 @default.
• W2068767522 cites W1963803322 @default.
• W2068767522 cites W1964569935 @default.
• W2068767522 cites W1965530530 @default.
• W2068767522 cites W1967056257 @default.
• W2068767522 cites W1977312502 @default.
• W2068767522 cites W1977473423 @default.
• W2068767522 cites W1984202431 @default.
• W2068767522 cites W1985097116 @default.
• W2068767522 cites W1993000713 @default.
• W2068767522 cites W2001641653 @default.
• W2068767522 cites W2002180552 @default.
• W2068767522 cites W2008362553 @default.
• W2068767522 cites W2010897185 @default.
• W2068767522 cites W2023260246 @default.
• W2068767522 cites W2024503898 @default.
• W2068767522 cites W2026395206 @default.
• W2068767522 cites W2028820703 @default.
• W2068767522 cites W2030517878 @default.
• W2068767522 cites W2031954037 @default.
• W2068767522 cites W2034767282 @default.
• W2068767522 cites W2053891116 @default.
• W2068767522 cites W2054326655 @default.
• W2068767522 cites W2057121401 @default.
• W2068767522 cites W2060955755 @default.
• W2068767522 cites W2080263879 @default.
• W2068767522 cites W2080510989 @default.
• W2068767522 cites W2084739795 @default.
• W2068767522 cites W2087098118 @default.
• W2068767522 cites W2094808915 @default.
• W2068767522 cites W2110397834 @default.
• W2068767522 cites W2134329421 @default.
• W2068767522 cites W2134816686 @default.
• W2068767522 cites W2144081223 @default.
• W2068767522 cites W2146080841 @default.
• W2068767522 cites W2149132502 @default.
• W2068767522 cites W2150527292 @default.
• W2068767522 cites W2154714625 @default.
• W2068767522 cites W2154802584 @default.
• W2068767522 cites W2159211495 @default.
• W2068767522 cites W2167393231 @default.
• W2068767522 cites W2188967191 @default.
• W2068767522 doi "https://doi.org/10.1016/j.jmb.2012.08.014" @default.
• W2068767522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22925580" @default.
• W2068767522 hasPublicationYear "2012" @default.
• W2068767522 type Work @default.
• W2068767522 sameAs 2068767522 @default.
• W2068767522 citedByCount "6" @default.
• W2068767522 countsByYear W20687675222013 @default.
• W2068767522 countsByYear W20687675222014 @default.
• W2068767522 countsByYear W20687675222022 @default.
• W2068767522 crossrefType "journal-article" @default.
• W2068767522 hasAuthorship W2068767522A5024598524 @default.
• W2068767522 hasAuthorship W2068767522A5025942956 @default.
• W2068767522 hasAuthorship W2068767522A5031761034 @default.
• W2068767522 hasAuthorship W2068767522A5054698608 @default.
• W2068767522 hasAuthorship W2068767522A5055625466 @default.
• W2068767522 hasAuthorship W2068767522A5062471072 @default.
• W2068767522 hasAuthorship W2068767522A5078498918 @default.
• W2068767522 hasConcept C104317684 @default.
• W2068767522 hasConcept C112887158 @default.
• W2068767522 hasConcept C178790620 @default.
• W2068767522 hasConcept C181199279 @default.
• W2068767522 hasConcept C185592680 @default.
• W2068767522 hasConcept C2776476023 @default.
• W2068767522 hasConcept C2776543447 @default.
• W2068767522 hasConcept C2779321679 @default.
• W2068767522 hasConcept C2779461342 @default.
• W2068767522 hasConcept C2780120296 @default.
• W2068767522 hasConcept C32909587 @default.
• W2068767522 hasConcept C33347731 @default.
• W2068767522 hasConcept C512185932 @default.
• W2068767522 hasConcept C55493867 @default.
• W2068767522 hasConcept C71240020 @default.
• W2068767522 hasConceptScore W2068767522C104317684 @default.
• W2068767522 hasConceptScore W2068767522C112887158 @default.
• W2068767522 hasConceptScore W2068767522C178790620 @default.
• W2068767522 hasConceptScore W2068767522C181199279 @default.
• W2068767522 hasConceptScore W2068767522C185592680 @default.
• W2068767522 hasConceptScore W2068767522C2776476023 @default.
• W2068767522 hasConceptScore W2068767522C2776543447 @default.
• W2068767522 hasConceptScore W2068767522C2779321679 @default.
• W2068767522 hasConceptScore W2068767522C2779461342 @default.
• W2068767522 hasConceptScore W2068767522C2780120296 @default.
• W2068767522 hasConceptScore W2068767522C32909587 @default.
• W2068767522 hasConceptScore W2068767522C33347731 @default.

• next