FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068779132> ?p ?o ?g. }

• W2068779132 endingPage "321" @default.
• W2068779132 startingPage "313" @default.
• W2068779132 abstract "Many quantitative approaches for analysis of helicase–nucleic acid interactions require a robust and specific signal, which reports on the presence of the helicase and its position on a nucleic acid lattice. Since 2006, iron–sulfur (FeS) clusters have been found in a number of helicases. They serve as endogenous quenchers of Cy3 and Cy5 fluorescence which can be exploited to characterize FeS cluster containing helicases both in ensemble-based assays and at the single-molecule level. Synthetic oligonucleotides site-specifically labeled with either Cy3 or Cy5 can be used to create a variety of DNA substrates that can be used to characterized DNA binding, as well as helicase translocation and unwinding. Equilibrium binding affinities for ssDNA, duplex and branched DNA substrates can be determined using bulk assays. Identification of preferred cognate substrates, and the orientation and position of the helicase when bound to DNA can also be determined by taking advantage of the intrinsic quencher in the helicase. At the single-molecule level, real-time observation of the helicase translocating along DNA either towards the dye or away from the dye can be used to determine the rate of translocation by the helicase on ssDNA and its orientation when bound to DNA. The use of duplex substrates can reveal the rate of unwinding and processivity of the helicase. Finally, the FeS cluster can be used to visualize protein–protein interactions, and to examine the interplay between helicases and other DNA binding proteins on the same DNA substrate." @default.
• W2068779132 created "2016-06-24" @default.
• W2068779132 creator A5008006973 @default.
• W2068779132 creator A5048385334 @default.
• W2068779132 creator A5077128553 @default.
• W2068779132 date "2010-07-01" @default.
• W2068779132 modified "2023-10-18" @default.
• W2068779132 title "Ensemble and single-molecule fluorescence-based assays to monitor DNA binding, translocation, and unwinding by iron–sulfur cluster containing helicases" @default.
• W2068779132 cites W1513995144 @default.
• W2068779132 cites W1637756689 @default.
• W2068779132 cites W1920913551 @default.
• W2068779132 cites W1967604909 @default.
• W2068779132 cites W1968747413 @default.
• W2068779132 cites W1969965681 @default.
• W2068779132 cites W1970449119 @default.
• W2068779132 cites W1984762921 @default.
• W2068779132 cites W1991703417 @default.
• W2068779132 cites W1994330097 @default.
• W2068779132 cites W2019502407 @default.
• W2068779132 cites W2021860235 @default.
• W2068779132 cites W2022133986 @default.
• W2068779132 cites W2031845110 @default.
• W2068779132 cites W2033788106 @default.
• W2068779132 cites W2060695776 @default.
• W2068779132 cites W2064853448 @default.
• W2068779132 cites W2070394525 @default.
• W2068779132 cites W2078951304 @default.
• W2068779132 cites W2079113974 @default.
• W2068779132 cites W2080380418 @default.
• W2068779132 cites W2090953848 @default.
• W2068779132 cites W2093173456 @default.
• W2068779132 cites W2094888311 @default.
• W2068779132 cites W2107090468 @default.
• W2068779132 cites W2113844848 @default.
• W2068779132 cites W2114382972 @default.
• W2068779132 cites W2115185917 @default.
• W2068779132 cites W2119119314 @default.
• W2068779132 cites W2122003098 @default.
• W2068779132 cites W2128479875 @default.
• W2068779132 cites W2130072201 @default.
• W2068779132 cites W2132768874 @default.
• W2068779132 cites W2141989464 @default.
• W2068779132 cites W2142844453 @default.
• W2068779132 cites W2156324690 @default.
• W2068779132 cites W2159669738 @default.
• W2068779132 cites W2166628163 @default.
• W2068779132 cites W2169594496 @default.
• W2068779132 cites W2399477397 @default.
• W2068779132 doi "https://doi.org/10.1016/j.ymeth.2010.02.014" @default.
• W2068779132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2911022" @default.
• W2068779132 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20167274" @default.
• W2068779132 hasPublicationYear "2010" @default.
• W2068779132 type Work @default.
• W2068779132 sameAs 2068779132 @default.
• W2068779132 citedByCount "16" @default.
• W2068779132 countsByYear W20687791322012 @default.
• W2068779132 countsByYear W20687791322013 @default.
• W2068779132 countsByYear W20687791322014 @default.
• W2068779132 countsByYear W20687791322015 @default.
• W2068779132 countsByYear W20687791322016 @default.
• W2068779132 countsByYear W20687791322021 @default.
• W2068779132 countsByYear W20687791322022 @default.
• W2068779132 countsByYear W20687791322023 @default.
• W2068779132 crossrefType "journal-article" @default.
• W2068779132 hasAuthorship W2068779132A5008006973 @default.
• W2068779132 hasAuthorship W2068779132A5048385334 @default.
• W2068779132 hasAuthorship W2068779132A5077128553 @default.
• W2068779132 hasBestOaLocation W20687791322 @default.
• W2068779132 hasConcept C104317684 @default.
• W2068779132 hasConcept C12554922 @default.
• W2068779132 hasConcept C129312508 @default.
• W2068779132 hasConcept C161223559 @default.
• W2068779132 hasConcept C181199279 @default.
• W2068779132 hasConcept C185592680 @default.
• W2068779132 hasConcept C24107716 @default.
• W2068779132 hasConcept C2777852564 @default.
• W2068779132 hasConcept C552990157 @default.
• W2068779132 hasConcept C55493867 @default.
• W2068779132 hasConcept C67705224 @default.
• W2068779132 hasConcept C68592252 @default.
• W2068779132 hasConcept C86803240 @default.
• W2068779132 hasConcept C99611785 @default.
• W2068779132 hasConceptScore W2068779132C104317684 @default.
• W2068779132 hasConceptScore W2068779132C12554922 @default.
• W2068779132 hasConceptScore W2068779132C129312508 @default.
• W2068779132 hasConceptScore W2068779132C161223559 @default.
• W2068779132 hasConceptScore W2068779132C181199279 @default.
• W2068779132 hasConceptScore W2068779132C185592680 @default.
• W2068779132 hasConceptScore W2068779132C24107716 @default.
• W2068779132 hasConceptScore W2068779132C2777852564 @default.
• W2068779132 hasConceptScore W2068779132C552990157 @default.
• W2068779132 hasConceptScore W2068779132C55493867 @default.
• W2068779132 hasConceptScore W2068779132C67705224 @default.
• W2068779132 hasConceptScore W2068779132C68592252 @default.
• W2068779132 hasConceptScore W2068779132C86803240 @default.
• W2068779132 hasConceptScore W2068779132C99611785 @default.
• W2068779132 hasIssue "3" @default.
• W2068779132 hasLocation W20687791321 @default.

• next