FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068787298> ?p ?o ?g. }

• W2068787298 endingPage "12853" @default.
• W2068787298 startingPage "12844" @default.
• W2068787298 abstract "Protein L-isoaspartyl methyltransferases (PIMT; EC 2.1.1.77) catalyze the S-adenosylmethionine-dependent methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide. In Drosophila melanogaster, overexpression of PIMT in transgenic flies extends the normal life span, suggesting that protein damage can be a limiting factor in longevity. To understand structural features of the Drosophila PIMT (dPIMT) important for catalysis, the crystal structure of dPIMT was determined at a resolution of 2.2 A, and site-directed mutagenesis was used to identify the role of Ser-60 in catalysis. The core structure of dPIMT is similar to the modified nucleotide-binding fold observed in PIMTs from extreme thermophiles and humans. A striking difference of the dPIMT structure is the rotation of the C-terminal residues by 90 degrees relative to the homologous structures. Effectively, this displacement generates a more open conformation that allows greater solvent access to S-adenosylhomocysteine, which is almost completely buried in other PIMT structures. The enzyme may alternate between the open conformation found for dPIMT and the more closed conformations described for other PIMTs during its catalytic cycle, thereby allowing the exchange of substrates and products. Catalysis by dPIMT requires the side chain of the conserved, active site residue Ser-60, since substitution of this residue with Thr, Gln, or Ala reduces or abolishes the methylation of both protein and isoaspartyl peptide substrates." @default.
• W2068787298 created "2016-06-24" @default.
• W2068787298 creator A5001819713 @default.
• W2068787298 creator A5015018913 @default.
• W2068787298 creator A5035591399 @default.
• W2068787298 creator A5042506993 @default.
• W2068787298 creator A5052352676 @default.
• W2068787298 creator A5088205477 @default.
• W2068787298 creator A5088598434 @default.
• W2068787298 date "2003-10-16" @default.
• W2068787298 modified "2023-10-10" @default.
• W2068787298 title "Catalytic Implications from the <i>Drosophila </i>Protein <scp>l</scp>-Isoaspartyl Methyltransferase Structure and Site-Directed Mutagenesis^," @default.
• W2068787298 cites W1498140959 @default.
• W2068787298 cites W1511355458 @default.
• W2068787298 cites W1524249696 @default.
• W2068787298 cites W1974436766 @default.
• W2068787298 cites W1980467950 @default.
• W2068787298 cites W1995017064 @default.
• W2068787298 cites W2001641653 @default.
• W2068787298 cites W2010096475 @default.
• W2068787298 cites W2029303304 @default.
• W2068787298 cites W2031338911 @default.
• W2068787298 cites W2045087373 @default.
• W2068787298 cites W2053720052 @default.
• W2068787298 cites W2056040984 @default.
• W2068787298 cites W2080983914 @default.
• W2068787298 cites W2081518835 @default.
• W2068787298 doi "https://doi.org/10.1021/bi034891+" @default.
• W2068787298 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14596598" @default.
• W2068787298 hasPublicationYear "2003" @default.
• W2068787298 type Work @default.
• W2068787298 sameAs 2068787298 @default.
• W2068787298 citedByCount "19" @default.
• W2068787298 countsByYear W20687872982012 @default.
• W2068787298 countsByYear W20687872982013 @default.
• W2068787298 countsByYear W20687872982015 @default.
• W2068787298 countsByYear W20687872982019 @default.
• W2068787298 countsByYear W20687872982020 @default.
• W2068787298 countsByYear W20687872982022 @default.
• W2068787298 crossrefType "journal-article" @default.
• W2068787298 hasAuthorship W2068787298A5001819713 @default.
• W2068787298 hasAuthorship W2068787298A5015018913 @default.
• W2068787298 hasAuthorship W2068787298A5035591399 @default.
• W2068787298 hasAuthorship W2068787298A5042506993 @default.
• W2068787298 hasAuthorship W2068787298A5052352676 @default.
• W2068787298 hasAuthorship W2068787298A5088205477 @default.
• W2068787298 hasAuthorship W2068787298A5088598434 @default.
• W2068787298 hasConcept C103408237 @default.
• W2068787298 hasConcept C104317684 @default.
• W2068787298 hasConcept C143065580 @default.
• W2068787298 hasConcept C16318435 @default.
• W2068787298 hasConcept C181199279 @default.
• W2068787298 hasConcept C185592680 @default.
• W2068787298 hasConcept C2779856020 @default.
• W2068787298 hasConcept C2780104201 @default.
• W2068787298 hasConcept C33288867 @default.
• W2068787298 hasConcept C41183919 @default.
• W2068787298 hasConcept C47701112 @default.
• W2068787298 hasConcept C515207424 @default.
• W2068787298 hasConcept C552990157 @default.
• W2068787298 hasConcept C55493867 @default.
• W2068787298 hasConcept C71240020 @default.
• W2068787298 hasConcept C91965660 @default.
• W2068787298 hasConceptScore W2068787298C103408237 @default.
• W2068787298 hasConceptScore W2068787298C104317684 @default.
• W2068787298 hasConceptScore W2068787298C143065580 @default.
• W2068787298 hasConceptScore W2068787298C16318435 @default.
• W2068787298 hasConceptScore W2068787298C181199279 @default.
• W2068787298 hasConceptScore W2068787298C185592680 @default.
• W2068787298 hasConceptScore W2068787298C2779856020 @default.
• W2068787298 hasConceptScore W2068787298C2780104201 @default.
• W2068787298 hasConceptScore W2068787298C33288867 @default.
• W2068787298 hasConceptScore W2068787298C41183919 @default.
• W2068787298 hasConceptScore W2068787298C47701112 @default.
• W2068787298 hasConceptScore W2068787298C515207424 @default.
• W2068787298 hasConceptScore W2068787298C552990157 @default.
• W2068787298 hasConceptScore W2068787298C55493867 @default.
• W2068787298 hasConceptScore W2068787298C71240020 @default.
• W2068787298 hasConceptScore W2068787298C91965660 @default.
• W2068787298 hasIssue "44" @default.
• W2068787298 hasLocation W20687872981 @default.
• W2068787298 hasLocation W20687872982 @default.
• W2068787298 hasOpenAccess W2068787298 @default.
• W2068787298 hasPrimaryLocation W20687872981 @default.
• W2068787298 hasRelatedWork W1488442929 @default.
• W2068787298 hasRelatedWork W1974177679 @default.
• W2068787298 hasRelatedWork W1974993927 @default.
• W2068787298 hasRelatedWork W2005680221 @default.
• W2068787298 hasRelatedWork W2023868084 @default.
• W2068787298 hasRelatedWork W2059519851 @default.
• W2068787298 hasRelatedWork W2062629157 @default.
• W2068787298 hasRelatedWork W2076615456 @default.
• W2068787298 hasRelatedWork W2082874149 @default.
• W2068787298 hasRelatedWork W294129591 @default.
• W2068787298 hasVolume "42" @default.
• W2068787298 isParatext "false" @default.
• W2068787298 isRetracted "false" @default.
• W2068787298 magId "2068787298" @default.

• next