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- W2068800181 abstract "Abstract The determination of transcellular transport characteristics using filter-grown brain capillary endothelial cells in conventional permeation studies requires extremely tight cell layers, exhibiting electrical resistance values of more than 500 ohm · cm2, for avoiding interferences with the paracellular flux. The present investigations demonstrate that rate and asymmetry of transcellular transport processes can be evaluated sufficiently reliably for orientation and comparison purposes also by use of more easily obtainable leaky cell layers exhibiting electrical resistance values of about 20 ohm · cm2 only. The studies, relying on measurements of rates for influx and efflux into and out of the cells, respectively, of the cell barrier were performed with aortic endothelial cell monolayers grown on permeable supports. Alanine, leucine, 3-(O-methyl)-D-glucose served as representative hydrophilic substrates being transported actively or by facilitated diffusion. HPLC, combined with a simple precolumn benzoylation appropriate for a simultaneous analysis of both substance groups, was used for the quantitation." @default.
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- W2068800181 date "1994-12-01" @default.
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- W2068800181 title "Utilization of endothelial cell monolayers of low tightness for estimation of transcellular transport characteristics of hydrophilic compounds" @default.
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- W2068800181 doi "https://doi.org/10.1016/0928-0987(94)00063-8" @default.
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