Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068807816> ?p ?o ?g. }
- W2068807816 endingPage "110" @default.
- W2068807816 startingPage "99" @default.
- W2068807816 abstract "The 51Cr release assay has traditionally been used to investigate effector cell cytotoxic function against labeled targets, but this method has inherent problems that include hazards associated with radioactivity, cell labeling and high spontaneous release. Here we describe a novel flow cytometric assay which addresses and improves upon the problems currently encountered with the 51Cr release assay. The fluorometric assessment of T lymphocyte antigen specific lysis (FATAL) assay employs dual staining (PKH-26 and CFSE) to identify and evaluate the target population. We found that the PKH-26/CFSE combination efficiently labeled target cells. Evaluation of the spontaneous leakage from dye labeled target cells was forty fold lower than the spontaneous leakage seen with the 51Cr release assay. The FATAL assay permitted a more accurate assessment of the effector: target ratio, and detected low levels of cytotoxic T lymphocyte (CTL) mediated lysis. There was a strong correlation between the 51Cr release and FATAL assays, when performed in parallel with identical effector and target cells (r(2)=0.998, P=<0.0001). This novel method of detecting cytolysis represents a qualitative and quantitative improvement over standard 51Cr release analysis. The FATAL assay will be of value to further investigate mechanisms of cytolysis by effector cell populations." @default.
- W2068807816 created "2016-06-24" @default.
- W2068807816 creator A5000950209 @default.
- W2068807816 creator A5020829925 @default.
- W2068807816 creator A5029546143 @default.
- W2068807816 creator A5055252960 @default.
- W2068807816 creator A5062617094 @default.
- W2068807816 date "2001-03-01" @default.
- W2068807816 modified "2023-09-28" @default.
- W2068807816 title "A novel technique for the fluorometric assessment of T lymphocyte antigen specific lysis" @default.
- W2068807816 cites W1202121494 @default.
- W2068807816 cites W120597284 @default.
- W2068807816 cites W139966805 @default.
- W2068807816 cites W1559828341 @default.
- W2068807816 cites W1587343486 @default.
- W2068807816 cites W1782472155 @default.
- W2068807816 cites W1857613114 @default.
- W2068807816 cites W1966092048 @default.
- W2068807816 cites W1966379453 @default.
- W2068807816 cites W1968706708 @default.
- W2068807816 cites W1969466196 @default.
- W2068807816 cites W1974967395 @default.
- W2068807816 cites W1985774274 @default.
- W2068807816 cites W1986170848 @default.
- W2068807816 cites W1986312885 @default.
- W2068807816 cites W1990034436 @default.
- W2068807816 cites W1991010069 @default.
- W2068807816 cites W1991751010 @default.
- W2068807816 cites W1992329276 @default.
- W2068807816 cites W1993159014 @default.
- W2068807816 cites W1998985248 @default.
- W2068807816 cites W1999577314 @default.
- W2068807816 cites W2000413691 @default.
- W2068807816 cites W2000933354 @default.
- W2068807816 cites W2003166335 @default.
- W2068807816 cites W2004183671 @default.
- W2068807816 cites W2007190082 @default.
- W2068807816 cites W2009645126 @default.
- W2068807816 cites W2011036671 @default.
- W2068807816 cites W2017301648 @default.
- W2068807816 cites W2020133075 @default.
- W2068807816 cites W2028316183 @default.
- W2068807816 cites W2029155502 @default.
- W2068807816 cites W2030792466 @default.
- W2068807816 cites W2031555958 @default.
- W2068807816 cites W2035703912 @default.
- W2068807816 cites W2037198595 @default.
- W2068807816 cites W2038113605 @default.
- W2068807816 cites W2042487497 @default.
- W2068807816 cites W2042606433 @default.
- W2068807816 cites W2048358606 @default.
- W2068807816 cites W2052936704 @default.
- W2068807816 cites W2054678965 @default.
- W2068807816 cites W2056021500 @default.
- W2068807816 cites W2058577087 @default.
- W2068807816 cites W2058935900 @default.
- W2068807816 cites W2059718955 @default.
- W2068807816 cites W2059995303 @default.
- W2068807816 cites W2060617067 @default.
- W2068807816 cites W2060932360 @default.
- W2068807816 cites W2060959197 @default.
- W2068807816 cites W2061064007 @default.
- W2068807816 cites W2064596281 @default.
- W2068807816 cites W2067069532 @default.
- W2068807816 cites W2072159436 @default.
- W2068807816 cites W2072179733 @default.
- W2068807816 cites W2072207738 @default.
- W2068807816 cites W2074550162 @default.
- W2068807816 cites W2074702304 @default.
- W2068807816 cites W2075609847 @default.
- W2068807816 cites W2077068404 @default.
- W2068807816 cites W2077314296 @default.
- W2068807816 cites W2078532650 @default.
- W2068807816 cites W2091148712 @default.
- W2068807816 cites W2092468599 @default.
- W2068807816 cites W2092792883 @default.
- W2068807816 cites W2093218866 @default.
- W2068807816 cites W2104417744 @default.
- W2068807816 cites W2110264618 @default.
- W2068807816 cites W2110374090 @default.
- W2068807816 cites W2123744397 @default.
- W2068807816 cites W2129114331 @default.
- W2068807816 cites W2138773604 @default.
- W2068807816 cites W2140237734 @default.
- W2068807816 cites W2144161089 @default.
- W2068807816 cites W2147181585 @default.
- W2068807816 cites W2149560370 @default.
- W2068807816 cites W2153136738 @default.
- W2068807816 cites W2159305020 @default.
- W2068807816 cites W2162330027 @default.
- W2068807816 cites W2165209977 @default.
- W2068807816 cites W2264208057 @default.
- W2068807816 cites W2325887155 @default.
- W2068807816 cites W40843633 @default.
- W2068807816 cites W6875991 @default.
- W2068807816 doi "https://doi.org/10.1016/s0022-1759(00)00329-x" @default.
- W2068807816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11226468" @default.
- W2068807816 hasPublicationYear "2001" @default.