FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068808437> ?p ?o ?g. }

• W2068808437 endingPage "3015" @default.
• W2068808437 startingPage "3009" @default.
• W2068808437 abstract "Abstract The Escherichia coli esterase YbfF displays high activity towards 1,2‐ O ‐isopropylideneglycerol (IPG) butyrate and IPG caprylate, and prefers the R ‐enantiomer of these substrates, producing the S ‐enantiomer of the IPG product in excess. To improve the potential of the enzyme for the kinetic resolution of racemic esters of IPG, an enhancement of the activity and enantioselectivity would be highly desirable. Molecular docking of the R ‐enantiomer of both IPG esters into the active site of YbfF allowed the identification of proximal YbfF active site residues. Four residues (25, 124, 185 and 235) were selected as targets for mutagenesis, in order to enhance YbfF activity and enantioselectivity towards IPG esters. Random mutagenesis at positions 25, 124, 185 and 235 yielded several best YbfF variants with enhanced activity and enantioselectivity towards IPG esters. The best YbfF mutant, W235I, exhibited a 2‐fold higher enantioselectivity than wild‐type YbfF, with an E =38 for IPG butyrate and an E =77 for IPG caprylate. Molecular docking experiments further support the enhanced enantioselectivity shown experimentally and the structural effects of this amino acid substitution on the active site of YbfF are provided. The engineered W235I mutant is an attractive catalyst for practical applications in the kinetic resolution of IPG esters." @default.
• W2068808437 created "2016-06-24" @default.
• W2068808437 creator A5006246608 @default.
• W2068808437 creator A5015832633 @default.
• W2068808437 creator A5030310908 @default.
• W2068808437 creator A5068183883 @default.
• W2068808437 creator A5079434594 @default.
• W2068808437 date "2012-11-04" @default.
• W2068808437 modified "2023-10-18" @default.
• W2068808437 title "An Esterase with Superior Activity and Enantioselectivity towards 1,2-<i>O</i>-Isopropylideneglycerol Esters Obtained by Protein Design" @default.
• W2068808437 cites W1971062326 @default.
• W2068808437 cites W1979025327 @default.
• W2068808437 cites W1983769595 @default.
• W2068808437 cites W1987489304 @default.
• W2068808437 cites W1987951295 @default.
• W2068808437 cites W1989281335 @default.
• W2068808437 cites W2015492208 @default.
• W2068808437 cites W2016596397 @default.
• W2068808437 cites W2026117532 @default.
• W2068808437 cites W2028161010 @default.
• W2068808437 cites W2035103700 @default.
• W2068808437 cites W2041210183 @default.
• W2068808437 cites W2045164740 @default.
• W2068808437 cites W2048432671 @default.
• W2068808437 cites W2063597962 @default.
• W2068808437 cites W2086462671 @default.
• W2068808437 cites W2091677383 @default.
• W2068808437 cites W2091883778 @default.
• W2068808437 cites W2094826641 @default.
• W2068808437 cites W2097985831 @default.
• W2068808437 cites W2106246537 @default.
• W2068808437 cites W2127478832 @default.
• W2068808437 cites W2145591075 @default.
• W2068808437 cites W2156053468 @default.
• W2068808437 cites W2166537396 @default.
• W2068808437 cites W37279920 @default.
• W2068808437 doi "https://doi.org/10.1002/adsc.201200211" @default.
• W2068808437 hasPublicationYear "2012" @default.
• W2068808437 type Work @default.
• W2068808437 sameAs 2068808437 @default.
• W2068808437 citedByCount "13" @default.
• W2068808437 countsByYear W20688084372013 @default.
• W2068808437 countsByYear W20688084372014 @default.
• W2068808437 countsByYear W20688084372015 @default.
• W2068808437 countsByYear W20688084372016 @default.
• W2068808437 countsByYear W20688084372017 @default.
• W2068808437 countsByYear W20688084372018 @default.
• W2068808437 countsByYear W20688084372019 @default.
• W2068808437 countsByYear W20688084372020 @default.
• W2068808437 crossrefType "journal-article" @default.
• W2068808437 hasAuthorship W2068808437A5006246608 @default.
• W2068808437 hasAuthorship W2068808437A5015832633 @default.
• W2068808437 hasAuthorship W2068808437A5030310908 @default.
• W2068808437 hasAuthorship W2068808437A5068183883 @default.
• W2068808437 hasAuthorship W2068808437A5079434594 @default.
• W2068808437 hasConcept C104317684 @default.
• W2068808437 hasConcept C143065580 @default.
• W2068808437 hasConcept C146686406 @default.
• W2068808437 hasConcept C159110408 @default.
• W2068808437 hasConcept C161790260 @default.
• W2068808437 hasConcept C178790620 @default.
• W2068808437 hasConcept C181199279 @default.
• W2068808437 hasConcept C185592680 @default.
• W2068808437 hasConcept C21951064 @default.
• W2068808437 hasConcept C2777261171 @default.
• W2068808437 hasConcept C28173026 @default.
• W2068808437 hasConcept C41183919 @default.
• W2068808437 hasConcept C41685203 @default.
• W2068808437 hasConcept C486523 @default.
• W2068808437 hasConcept C55493867 @default.
• W2068808437 hasConcept C71240020 @default.
• W2068808437 hasConcept C71924100 @default.
• W2068808437 hasConcept C9418097 @default.
• W2068808437 hasConceptScore W2068808437C104317684 @default.
• W2068808437 hasConceptScore W2068808437C143065580 @default.
• W2068808437 hasConceptScore W2068808437C146686406 @default.
• W2068808437 hasConceptScore W2068808437C159110408 @default.
• W2068808437 hasConceptScore W2068808437C161790260 @default.
• W2068808437 hasConceptScore W2068808437C178790620 @default.
• W2068808437 hasConceptScore W2068808437C181199279 @default.
• W2068808437 hasConceptScore W2068808437C185592680 @default.
• W2068808437 hasConceptScore W2068808437C21951064 @default.
• W2068808437 hasConceptScore W2068808437C2777261171 @default.
• W2068808437 hasConceptScore W2068808437C28173026 @default.
• W2068808437 hasConceptScore W2068808437C41183919 @default.
• W2068808437 hasConceptScore W2068808437C41685203 @default.
• W2068808437 hasConceptScore W2068808437C486523 @default.
• W2068808437 hasConceptScore W2068808437C55493867 @default.
• W2068808437 hasConceptScore W2068808437C71240020 @default.
• W2068808437 hasConceptScore W2068808437C71924100 @default.
• W2068808437 hasConceptScore W2068808437C9418097 @default.
• W2068808437 hasIssue "16" @default.
• W2068808437 hasLocation W20688084371 @default.
• W2068808437 hasOpenAccess W2068808437 @default.
• W2068808437 hasPrimaryLocation W20688084371 @default.
• W2068808437 hasRelatedWork W1604672294 @default.
• W2068808437 hasRelatedWork W1994240720 @default.
• W2068808437 hasRelatedWork W2027084378 @default.

• next