FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068856689> ?p ?o ?g. }

• W2068856689 endingPage "1217" @default.
• W2068856689 startingPage "1205" @default.
• W2068856689 abstract "Wnt/β-catenin signaling is an important regulator of cell polarity, proliferation, and stem cell maintenance during development and adulthood. Wnt proteins induce the nuclear accumulation of β-catenin, which regulates the expression of Wnt-responsive genes through association with TCF/LEF transcription factors. Aberrant Wnt/β-catenin signaling has been implicated in a plethora of pathologies and, most notably, underlies initiation and expansion of several cancers. Here, we apply enzyme fragment complementation to measure the nuclear accumulation of β-catenin. β-Catenin was tagged with a peptide fragment of β-galactosidase and transfected into cells expressing a corresponding deletion mutant of the enzyme exclusively in the nucleus. Stimulation of the cells with recombinant Wnt-3a restored β-galactosidase activity in a dose-dependent manner with nanomolar potency. Using the assay, we confirmed that Wnt-5a represses β-catenin-driven reporter gene activity downstream of nuclear entry of β-catenin. In addition, we tested a library of >2000 synthetic chemical compounds for their ability to induce β-catenin nuclear accumulation. The immunosuppressive protein kinase C inhibitor so-trastaurin (AEB-071) was identified as an activator of Wnt/β-catenin signaling at micromolar concentrations. It was confirmed that the compound stabilizes endogenous β-catenin protein and can induce TCF/LEF-dependent gene transcription. Subsequent biochemical profiling of > 200 kinases revealed both isoforms of glycogen synthase kinase 3, as previously unappreciated targets of so-trastaurin. We show that the β-catenin nuclear accumulation assay contributes to our knowledge of molecular interactions within the Wnt/β-catenin pathway and can be used to find new therapeutics targeting Wnt/β-catenin signaling.—Verkaar, F., Blankesteijn, W. M., Smits, J. F. M., Zaman, G. J. R. β-Galactosidase enzyme fragment complementation for the measurement of Wnt/β-catenin signaling. FASEB J. 24, 1205–1217 (2010). www.fasebj.org" @default.
• W2068856689 created "2016-06-24" @default.
• W2068856689 creator A5008629639 @default.
• W2068856689 creator A5035889183 @default.
• W2068856689 creator A5073444042 @default.
• W2068856689 creator A5086196292 @default.
• W2068856689 date "2009-11-25" @default.
• W2068856689 modified "2023-10-13" @default.
• W2068856689 title "β‐Galactosidase enzyme fragment complementation for the measurement of Wnt/β‐catenin signaling" @default.
• W2068856689 cites W1532171869 @default.
• W2068856689 cites W1546114439 @default.
• W2068856689 cites W1963819880 @default.
• W2068856689 cites W1967799178 @default.
• W2068856689 cites W1969717239 @default.
• W2068856689 cites W1975454216 @default.
• W2068856689 cites W1982219886 @default.
• W2068856689 cites W1990521388 @default.
• W2068856689 cites W1991001144 @default.
• W2068856689 cites W2008744347 @default.
• W2068856689 cites W2009684443 @default.
• W2068856689 cites W2025394006 @default.
• W2068856689 cites W2032112650 @default.
• W2068856689 cites W2032619610 @default.
• W2068856689 cites W2033794836 @default.
• W2068856689 cites W2035487460 @default.
• W2068856689 cites W2041572913 @default.
• W2068856689 cites W2050112710 @default.
• W2068856689 cites W2051788298 @default.
• W2068856689 cites W2087513748 @default.
• W2068856689 cites W2090636859 @default.
• W2068856689 cites W2091258025 @default.
• W2068856689 cites W2099624082 @default.
• W2068856689 cites W2100273052 @default.
• W2068856689 cites W2101889783 @default.
• W2068856689 cites W2104204293 @default.
• W2068856689 cites W2107648723 @default.
• W2068856689 cites W2111785842 @default.
• W2068856689 cites W2117022661 @default.
• W2068856689 cites W2119284420 @default.
• W2068856689 cites W2119780609 @default.
• W2068856689 cites W2128237690 @default.
• W2068856689 cites W2139196151 @default.
• W2068856689 cites W2146812359 @default.
• W2068856689 cites W2147257521 @default.
• W2068856689 cites W2154041910 @default.
• W2068856689 cites W2163279201 @default.
• W2068856689 doi "https://doi.org/10.1096/fj.09-141671" @default.
• W2068856689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19940259" @default.
• W2068856689 hasPublicationYear "2009" @default.
• W2068856689 type Work @default.
• W2068856689 sameAs 2068856689 @default.
• W2068856689 citedByCount "20" @default.
• W2068856689 countsByYear W20688566892012 @default.
• W2068856689 countsByYear W20688566892013 @default.
• W2068856689 countsByYear W20688566892014 @default.
• W2068856689 countsByYear W20688566892015 @default.
• W2068856689 countsByYear W20688566892016 @default.
• W2068856689 crossrefType "journal-article" @default.
• W2068856689 hasAuthorship W2068856689A5008629639 @default.
• W2068856689 hasAuthorship W2068856689A5035889183 @default.
• W2068856689 hasAuthorship W2068856689A5073444042 @default.
• W2068856689 hasAuthorship W2068856689A5086196292 @default.
• W2068856689 hasBestOaLocation W20688566892 @default.
• W2068856689 hasConcept C104317684 @default.
• W2068856689 hasConcept C137620995 @default.
• W2068856689 hasConcept C153911025 @default.
• W2068856689 hasConcept C182819311 @default.
• W2068856689 hasConcept C182996813 @default.
• W2068856689 hasConcept C185592680 @default.
• W2068856689 hasConcept C2779324961 @default.
• W2068856689 hasConcept C2780723820 @default.
• W2068856689 hasConcept C30145163 @default.
• W2068856689 hasConcept C49805395 @default.
• W2068856689 hasConcept C54009773 @default.
• W2068856689 hasConcept C55493867 @default.
• W2068856689 hasConcept C62478195 @default.
• W2068856689 hasConcept C86803240 @default.
• W2068856689 hasConcept C95444343 @default.
• W2068856689 hasConceptScore W2068856689C104317684 @default.
• W2068856689 hasConceptScore W2068856689C137620995 @default.
• W2068856689 hasConceptScore W2068856689C153911025 @default.
• W2068856689 hasConceptScore W2068856689C182819311 @default.
• W2068856689 hasConceptScore W2068856689C182996813 @default.
• W2068856689 hasConceptScore W2068856689C185592680 @default.
• W2068856689 hasConceptScore W2068856689C2779324961 @default.
• W2068856689 hasConceptScore W2068856689C2780723820 @default.
• W2068856689 hasConceptScore W2068856689C30145163 @default.
• W2068856689 hasConceptScore W2068856689C49805395 @default.
• W2068856689 hasConceptScore W2068856689C54009773 @default.
• W2068856689 hasConceptScore W2068856689C55493867 @default.
• W2068856689 hasConceptScore W2068856689C62478195 @default.
• W2068856689 hasConceptScore W2068856689C86803240 @default.
• W2068856689 hasConceptScore W2068856689C95444343 @default.
• W2068856689 hasFunder F4320309111 @default.
• W2068856689 hasFunder F4320310094 @default.
• W2068856689 hasIssue "4" @default.
• W2068856689 hasLocation W20688566891 @default.
• W2068856689 hasLocation W20688566892 @default.

• next