FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068863414> ?p ?o ?g. }

• W2068863414 endingPage "5765" @default.
• W2068863414 startingPage "5757" @default.
• W2068863414 abstract "Interactions of cationic antimicrobial peptides with living bacterial and mammalian cells are little understood, although model membranes have been used extensively to elucidate how peptides permeabilize membranes. In this study, the interaction of F5W-magainin 2 (GIGKWLHSAKKFGKAFVGEIMNS), an equipotent analogue of magainin 2 isolated from the African clawed frog Xenopus laevis, with unfixed Bacillus megaterium and Chinese hamster ovary (CHO)-K1 cells was investigated, using confocal laser scanning microscopy. A small amount of tetramethylrhodamine-labeled F5W-magainin 2 was incorporated into the unlabeled peptide for imaging. The influx of fluorescent markers of various sizes into the cytosol revealed that magainin 2 permeabilized bacterial and mammalian membranes in significantly different ways. The peptide formed pores with a diameter of ∼2.8 nm (< 6.6 nm) in B. megaterium, and translocated into the cytosol. In contrast, the peptide significantly perturbed the membrane of CHO-K1 cells, permitting the entry of a large molecule (diameter, >23 nm) into the cytosol, accompanied by membrane budding and lipid flip-flop, mainly accumulating in mitochondria and nuclei. Adenosine triphosphate and negatively charged glycosaminoglycans were little involved in the magainin-induced permeabilization of membranes in CHO-K1 cells. Furthermore, the susceptibility of CHO-K1 cells to magainin was found to be similar to that of erythrocytes. Thus, the distinct membrane-permeabilizing processes of magainin 2 in bacterial and mammalian cells were, to the best of our knowledge, visualized and characterized in detail for the first time." @default.
• W2068863414 created "2016-06-24" @default.
• W2068863414 creator A5012808950 @default.
• W2068863414 creator A5050805758 @default.
• W2068863414 creator A5068024490 @default.
• W2068863414 date "2008-12-01" @default.
• W2068863414 modified "2023-10-11" @default.
• W2068863414 title "Magainin 2 in Action: Distinct Modes of Membrane Permeabilization in Living Bacterial and Mammalian Cells" @default.
• W2068863414 cites W1490172408 @default.
• W2068863414 cites W1964436136 @default.
• W2068863414 cites W1975967141 @default.
• W2068863414 cites W1976258107 @default.
• W2068863414 cites W1978173156 @default.
• W2068863414 cites W1979049228 @default.
• W2068863414 cites W1981312603 @default.
• W2068863414 cites W1988884953 @default.
• W2068863414 cites W1989557252 @default.
• W2068863414 cites W1990233153 @default.
• W2068863414 cites W1990404775 @default.
• W2068863414 cites W1993791807 @default.
• W2068863414 cites W1996908859 @default.
• W2068863414 cites W1997152791 @default.
• W2068863414 cites W1997947008 @default.
• W2068863414 cites W2001038738 @default.
• W2068863414 cites W2002288900 @default.
• W2068863414 cites W2002416967 @default.
• W2068863414 cites W2004926324 @default.
• W2068863414 cites W2005656463 @default.
• W2068863414 cites W2006008435 @default.
• W2068863414 cites W2007744639 @default.
• W2068863414 cites W2008931830 @default.
• W2068863414 cites W2009440109 @default.
• W2068863414 cites W2013092938 @default.
• W2068863414 cites W2013418133 @default.
• W2068863414 cites W2016809324 @default.
• W2068863414 cites W2031701155 @default.
• W2068863414 cites W2032368629 @default.
• W2068863414 cites W2038312689 @default.
• W2068863414 cites W2054602599 @default.
• W2068863414 cites W2055372535 @default.
• W2068863414 cites W2061784494 @default.
• W2068863414 cites W2064282122 @default.
• W2068863414 cites W2070957092 @default.
• W2068863414 cites W2071581047 @default.
• W2068863414 cites W2071737749 @default.
• W2068863414 cites W2073097129 @default.
• W2068863414 cites W2073894126 @default.
• W2068863414 cites W2079468421 @default.
• W2068863414 cites W2081088550 @default.
• W2068863414 cites W2086264237 @default.
• W2068863414 cites W2092699435 @default.
• W2068863414 cites W2131909172 @default.
• W2068863414 cites W2136840366 @default.
• W2068863414 cites W2139069865 @default.
• W2068863414 cites W2147531177 @default.
• W2068863414 cites W2147533168 @default.
• W2068863414 cites W2150668442 @default.
• W2068863414 cites W215423543 @default.
• W2068863414 cites W2158261605 @default.
• W2068863414 cites W2158482458 @default.
• W2068863414 cites W2161673554 @default.
• W2068863414 cites W2166291746 @default.
• W2068863414 cites W2397887121 @default.
• W2068863414 cites W4235982002 @default.
• W2068863414 doi "https://doi.org/10.1529/biophysj.108.133488" @default.
• W2068863414 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2599864" @default.
• W2068863414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18835901" @default.
• W2068863414 hasPublicationYear "2008" @default.
• W2068863414 type Work @default.
• W2068863414 sameAs 2068863414 @default.
• W2068863414 citedByCount "98" @default.
• W2068863414 countsByYear W20688634142012 @default.
• W2068863414 countsByYear W20688634142013 @default.
• W2068863414 countsByYear W20688634142014 @default.
• W2068863414 countsByYear W20688634142015 @default.
• W2068863414 countsByYear W20688634142016 @default.
• W2068863414 countsByYear W20688634142017 @default.
• W2068863414 countsByYear W20688634142018 @default.
• W2068863414 countsByYear W20688634142019 @default.
• W2068863414 countsByYear W20688634142020 @default.
• W2068863414 countsByYear W20688634142021 @default.
• W2068863414 countsByYear W20688634142022 @default.
• W2068863414 countsByYear W20688634142023 @default.
• W2068863414 crossrefType "journal-article" @default.
• W2068863414 hasAuthorship W2068863414A5012808950 @default.
• W2068863414 hasAuthorship W2068863414A5050805758 @default.
• W2068863414 hasAuthorship W2068863414A5068024490 @default.
• W2068863414 hasBestOaLocation W20688634141 @default.
• W2068863414 hasConcept C104317684 @default.
• W2068863414 hasConcept C12554922 @default.
• W2068863414 hasConcept C170493617 @default.
• W2068863414 hasConcept C175656101 @default.
• W2068863414 hasConcept C181199279 @default.
• W2068863414 hasConcept C185592680 @default.
• W2068863414 hasConcept C2778553168 @default.
• W2068863414 hasConcept C2779281246 @default.
• W2068863414 hasConcept C2779535977 @default.
• W2068863414 hasConcept C41625074 @default.

• next