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- W2068885826 abstract "Abstract The Cerrado biome represents a hotspot of biodiversity. Despite this, the nematofauna in this biome has not been well characterized, especially that related to root‐knot nematodes. This work aimed to identify M eloidogyne species present in different cerrado vegetations and to investigate potential hosts of M eloidogyne javanica in this biome. Soil samples (250) were collected in native areas of cerrado vegetation located at the National Park of Brasília ( PNB ) (125 samples) and Água Limpa Farm ( FAL ) (125 samples), and transferred to sterile pots. Single tomato plants cv. Santa Clara (susceptible) were transplanted into individual pots and maintained for 90 days under glasshouse. Females of M eloidogyne spp. were extracted from tomato roots and identified based upon esterase phenotypes and confirmed with PCR using specific sequence characterized amplified regions ( SCAR ) primers. Native plants were inoculated with 10 000 individuals (eggs + J2) of a pure culture of M . javanica and maintained under glasshouse for 6 months. From the 250 samples collected, 57 (22.8%) presented M eloidogyne spp. A total of 66 Meloidogyne populations were identified as follows: M . javanica (75.76%), M . incognita (10.60%), M . hapla (9.1%), M . morocciensis (3.03%) and M . arenaria (1.51%). The following esterase phenotypes were detected: M . javanica (J3 and J2), M . incognita (I1 and I2), M . hapla (H1), M . morocciensis (A3) and M . arenaria (A2). The SCAR primers incK14F/incK14R, Fjav/Rjav and Fh/Rh amplified specific fragments in M . incognita (399 bp), M . javanica (670 bp) and M . hapla (610 bp) and can be used for identification of indigenous M eloidogyne spp. from cerrado. The primer set Far/Rar is not specific for M . arenaria due to the amplification of DNA in M . morocciensis . M imosa caesalpiniifolia was the only native plant in which M . javanica developed a high reproductive rate, and it is probably a host for this nematode in cerrado." @default.
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- W2068885826 date "2013-12-27" @default.
- W2068885826 modified "2023-10-03" @default.
- W2068885826 title "Occurrence of <i>Meloidogyne</i> spp. in Cerrado Vegetations and Reaction of Native Plants to <i>Meloidogyne javanica</i>" @default.
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- W2068885826 doi "https://doi.org/10.1111/jph.12211" @default.
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