FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068928097> ?p ?o ?g. }

• W2068928097 endingPage "550" @default.
• W2068928097 startingPage "541" @default.
• W2068928097 abstract "A flow-cytometric method was developed and evaluated as a rapid ecotoxicological tool using cultures of the microalga Chlamydomonas reinhardtii (Chlorophyceae) under cadmium exposure. Three staining protocols were developed to assess the toxicological impact of this trace metal on algal physiology. Algal cells were exposed to total nominal cadmium concentrations of 5 and 100 µM. After 48 and 72 h exposure the fluorescent probes, fluorescein diacetate (FDA), dihydrorhodamine 123 (DHR123) and tetramethylrhodamine methyl ester (TMRM), were used to assess esterase activity, presence of reactive oxygen species and membrane potential, respectively. Results indicated that cell size, cell granularity and internal complexity were influenced by cadmium, confirming earlier findings on ultrastructural changes in microalgae exposed to trace metals. An increase was observed in the percentage of DHR123 positive cells as well as in their mean fluorescence intensity, on increasing cadmium concentration, confirming that this metal exerts its toxicity through the generation of reactive oxygen species. Furthermore, cadmium exposure resulted in an increase in esterase activity, as reflected in fluorescein fluorescence. We suggest this observation was linked to possible detoxification activity and defence mechanisms. Measurements of control samples during protocol optimization for TMRM proved not to be reproducible, leading us to defer any judgment on results of exposed samples and to conclude that TMRM does not seem suitable for flow cytometric use in algae. Our results demonstrate that although very rarely used in ecotoxicology, flow cytometry is a quick and convenient technique to assess toxic effects that can generate mechanistic information on the mode of action of contaminants." @default.
• W2068928097 created "2016-06-24" @default.
• W2068928097 creator A5022150674 @default.
• W2068928097 creator A5044110545 @default.
• W2068928097 creator A5054036022 @default.
• W2068928097 creator A5063391728 @default.
• W2068928097 creator A5064650050 @default.
• W2068928097 creator A5080111340 @default.
• W2068928097 date "2009-11-01" @default.
• W2068928097 modified "2023-09-23" @default.
• W2068928097 title "Flow cytometric analysis of the cadmium-exposed green alga<i>Chlamydomonas reinhardtii</i>(Chlorophyceae)" @default.
• W2068928097 cites W1751026903 @default.
• W2068928097 cites W1970940158 @default.
• W2068928097 cites W1978027778 @default.
• W2068928097 cites W1981597415 @default.
• W2068928097 cites W1984888820 @default.
• W2068928097 cites W1985825236 @default.
• W2068928097 cites W1989981983 @default.
• W2068928097 cites W1991656295 @default.
• W2068928097 cites W1996266267 @default.
• W2068928097 cites W2006547543 @default.
• W2068928097 cites W2009388735 @default.
• W2068928097 cites W2010042676 @default.
• W2068928097 cites W2015869197 @default.
• W2068928097 cites W2020138346 @default.
• W2068928097 cites W2021789309 @default.
• W2068928097 cites W2022194175 @default.
• W2068928097 cites W2024431555 @default.
• W2068928097 cites W2032244596 @default.
• W2068928097 cites W2033285747 @default.
• W2068928097 cites W2047284046 @default.
• W2068928097 cites W2047549264 @default.
• W2068928097 cites W2050125347 @default.
• W2068928097 cites W2059459858 @default.
• W2068928097 cites W2061496487 @default.
• W2068928097 cites W2062158175 @default.
• W2068928097 cites W2067249601 @default.
• W2068928097 cites W2071829959 @default.
• W2068928097 cites W2081181593 @default.
• W2068928097 cites W2083020576 @default.
• W2068928097 cites W2087737359 @default.
• W2068928097 cites W2088078023 @default.
• W2068928097 cites W2088369610 @default.
• W2068928097 cites W2089663038 @default.
• W2068928097 cites W2090116867 @default.
• W2068928097 cites W2095184945 @default.
• W2068928097 cites W2099021096 @default.
• W2068928097 cites W2129696634 @default.
• W2068928097 cites W2146890671 @default.
• W2068928097 cites W2152783201 @default.
• W2068928097 cites W2161513935 @default.
• W2068928097 cites W4252783316 @default.
• W2068928097 doi "https://doi.org/10.1080/09670260903118214" @default.
• W2068928097 hasPublicationYear "2009" @default.
• W2068928097 type Work @default.
• W2068928097 sameAs 2068928097 @default.
• W2068928097 citedByCount "70" @default.
• W2068928097 countsByYear W20689280972012 @default.
• W2068928097 countsByYear W20689280972013 @default.
• W2068928097 countsByYear W20689280972014 @default.
• W2068928097 countsByYear W20689280972015 @default.
• W2068928097 countsByYear W20689280972016 @default.
• W2068928097 countsByYear W20689280972017 @default.
• W2068928097 countsByYear W20689280972018 @default.
• W2068928097 countsByYear W20689280972019 @default.
• W2068928097 countsByYear W20689280972020 @default.
• W2068928097 countsByYear W20689280972021 @default.
• W2068928097 countsByYear W20689280972022 @default.
• W2068928097 countsByYear W20689280972023 @default.
• W2068928097 crossrefType "journal-article" @default.
• W2068928097 hasAuthorship W2068928097A5022150674 @default.
• W2068928097 hasAuthorship W2068928097A5044110545 @default.
• W2068928097 hasAuthorship W2068928097A5054036022 @default.
• W2068928097 hasAuthorship W2068928097A5063391728 @default.
• W2068928097 hasAuthorship W2068928097A5064650050 @default.
• W2068928097 hasAuthorship W2068928097A5080111340 @default.
• W2068928097 hasBestOaLocation W20689280971 @default.
• W2068928097 hasConcept C104317684 @default.
• W2068928097 hasConcept C107872376 @default.
• W2068928097 hasConcept C115346097 @default.
• W2068928097 hasConcept C12554922 @default.
• W2068928097 hasConcept C143065580 @default.
• W2068928097 hasConcept C153911025 @default.
• W2068928097 hasConcept C178790620 @default.
• W2068928097 hasConcept C185592680 @default.
• W2068928097 hasConcept C2776580309 @default.
• W2068928097 hasConcept C2777550515 @default.
• W2068928097 hasConcept C2777563530 @default.
• W2068928097 hasConcept C2777727519 @default.
• W2068928097 hasConcept C33070731 @default.
• W2068928097 hasConcept C48349386 @default.
• W2068928097 hasConcept C544657597 @default.
• W2068928097 hasConcept C553184892 @default.
• W2068928097 hasConcept C55493867 @default.
• W2068928097 hasConcept C559758991 @default.
• W2068928097 hasConcept C59822182 @default.
• W2068928097 hasConcept C86803240 @default.
• W2068928097 hasConceptScore W2068928097C104317684 @default.

• next