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- W2068939449 abstract "Abstract The seeds of Psophocarpus scandens contain two distinct groups of lectins. The lectins adsorbed by melibiose Bio-Gel P150 yield two distinct components (lectins B1 and B2) on gel filtration on Ultrogel AcA 44. Both are glycoproteins (∼ 9% carbohydrate) M r ∼ 62 000, composed of two non-covalently bound subunits with isoelectric points (pl range 5.9–6.7) much lower than the corresponding melibiose adsorbed lectins from Psophocarpus tetragonolobus (winged bean). Isoclectric focusing revealed the presence of subunit charge heterogeneity resulting in several isolectin forms. The N -terminal sequences of the lectins were unique and showed extensive homology with other Psophocarpus and legume lectins. The two lectins were distinguished by different carbohydrate and erythrocyte specificities. Lectin B1 was inhibited by α-D-galactosides, such as melibiose, but not β- d -galactosides, such as lactose, and it preferentially agglutinated trypsinized rabbit erythrocytes while trypsinized human type O erythrocytes were very weakly agglutinated. Lectin B2 was equally well inhibited by both α- d -galactosides and α- d -galactosides, and agglutinated trypsinized rabbit and human type (A, B, O) erythrocytes to the same extent. The lectins not adsorbed by the melibiose Bio-Gel P1 50 were purified by chromatgraphy on Ultrogel AcA 44 and SP-Sephadex C-25 to yield three major components. These lectins are also glycoproteins (∼6% carbohydrate), M r ∼60 000 composed of two subunits. SDS-PAGE gave subunits of varying sizes ( M r 32 500–35 500) which showed little charge heterogeneity. The amino acid compositions and N -terminal sequences showed that these lectins are closely related to the acidic lectins of P. tetragonolobus . Furthermore, their erythrocyte haemagglutinating and sugar specificities are very similar to those of the acidic lectins of P. tetragonolobus ." @default.
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- W2068939449 date "1988-01-01" @default.
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- W2068939449 title "Isolation and characterization of the lectins from the seeds of Psophocarpus scandens" @default.
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- W2068939449 doi "https://doi.org/10.1016/0031-9422(88)80675-7" @default.
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