Matches in SemOpenAlex for { <https://semopenalex.org/work/W2068959365> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2068959365 endingPage "349" @default.
- W2068959365 startingPage "343" @default.
- W2068959365 abstract "Automated column-switching HPLC methods were developed and utilized for the direct analyses of three hydroxamic acid based metalloprotease inhibitors in rat plasma. These column-switching methods involved the use of a restricted-access media (RAM) precolumn and a column-switching valve, allowing the complete automation of sample preparation and HPLC. The plasma samples were directly injected onto a precolumn packed with SPS/ODS stationary phase and then backflushed onto an ODS analytical column using a 6-port column-switching device. The drug stability in rat plasma was determined using both the automated and traditional HPLC methods. The results obtained from the automated column-switching methods were in good agreement with those from traditional methods that involve sequential protein precipitation, liquid extraction, solvent evaporation, and sample reconstitution. In addition to the elimination of labor-intensive and time-consuming sample preparation procedures, the column-switching methods allowed on-line analyte enrichment and accurate determination of drug stability in plasma with detection limits in the range of 10-20 ng/ml(-1). This work represents, for the first time, a drug stability study in plasma by automated column-switching HPLC technique with the use of a RAM column. Our column-switching methods can be readily adapted to any existing HPLC system with minimal hardware modification." @default.
- W2068959365 created "2016-06-24" @default.
- W2068959365 creator A5003135016 @default.
- W2068959365 creator A5055367183 @default.
- W2068959365 creator A5065236994 @default.
- W2068959365 date "1999-03-01" @default.
- W2068959365 modified "2023-09-23" @default.
- W2068959365 title "Direct determination of stability of protease inhibitors in plasma by HPLC with automated column-switching" @default.
- W2068959365 cites W173584574 @default.
- W2068959365 cites W1975890572 @default.
- W2068959365 cites W1981193497 @default.
- W2068959365 cites W1996262571 @default.
- W2068959365 cites W1996970036 @default.
- W2068959365 cites W2005069429 @default.
- W2068959365 cites W2046043223 @default.
- W2068959365 cites W2056427715 @default.
- W2068959365 cites W2067545176 @default.
- W2068959365 cites W2080881393 @default.
- W2068959365 cites W2082662891 @default.
- W2068959365 cites W2092291949 @default.
- W2068959365 cites W2092719343 @default.
- W2068959365 doi "https://doi.org/10.1016/s0731-7085(98)00127-7" @default.
- W2068959365 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10704100" @default.
- W2068959365 hasPublicationYear "1999" @default.
- W2068959365 type Work @default.
- W2068959365 sameAs 2068959365 @default.
- W2068959365 citedByCount "14" @default.
- W2068959365 countsByYear W20689593652012 @default.
- W2068959365 countsByYear W20689593652013 @default.
- W2068959365 countsByYear W20689593652014 @default.
- W2068959365 countsByYear W20689593652016 @default.
- W2068959365 crossrefType "journal-article" @default.
- W2068959365 hasAuthorship W2068959365A5003135016 @default.
- W2068959365 hasAuthorship W2068959365A5055367183 @default.
- W2068959365 hasAuthorship W2068959365A5065236994 @default.
- W2068959365 hasConcept C10390740 @default.
- W2068959365 hasConcept C127413603 @default.
- W2068959365 hasConcept C13355873 @default.
- W2068959365 hasConcept C179998833 @default.
- W2068959365 hasConcept C185592680 @default.
- W2068959365 hasConcept C2778576202 @default.
- W2068959365 hasConcept C2780551164 @default.
- W2068959365 hasConcept C43617362 @default.
- W2068959365 hasConcept C66938386 @default.
- W2068959365 hasConcept C74774607 @default.
- W2068959365 hasConceptScore W2068959365C10390740 @default.
- W2068959365 hasConceptScore W2068959365C127413603 @default.
- W2068959365 hasConceptScore W2068959365C13355873 @default.
- W2068959365 hasConceptScore W2068959365C179998833 @default.
- W2068959365 hasConceptScore W2068959365C185592680 @default.
- W2068959365 hasConceptScore W2068959365C2778576202 @default.
- W2068959365 hasConceptScore W2068959365C2780551164 @default.
- W2068959365 hasConceptScore W2068959365C43617362 @default.
- W2068959365 hasConceptScore W2068959365C66938386 @default.
- W2068959365 hasConceptScore W2068959365C74774607 @default.
- W2068959365 hasIssue "3-4" @default.
- W2068959365 hasLocation W20689593651 @default.
- W2068959365 hasLocation W20689593652 @default.
- W2068959365 hasOpenAccess W2068959365 @default.
- W2068959365 hasPrimaryLocation W20689593651 @default.
- W2068959365 hasRelatedWork W1973892552 @default.
- W2068959365 hasRelatedWork W1990545609 @default.
- W2068959365 hasRelatedWork W2000950530 @default.
- W2068959365 hasRelatedWork W2002567025 @default.
- W2068959365 hasRelatedWork W2030312175 @default.
- W2068959365 hasRelatedWork W2078079779 @default.
- W2068959365 hasRelatedWork W2090751845 @default.
- W2068959365 hasRelatedWork W2156377834 @default.
- W2068959365 hasRelatedWork W2809682864 @default.
- W2068959365 hasRelatedWork W2520203833 @default.
- W2068959365 hasVolume "19" @default.
- W2068959365 isParatext "false" @default.
- W2068959365 isRetracted "false" @default.
- W2068959365 magId "2068959365" @default.
- W2068959365 workType "article" @default.