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• W2068960211 abstract "In this work we have studied the kinetic parameters of oligomeric tumour necrosis factor α (TNF-α) dissociation using biospecific interaction analysis (BIA), based on surface plasmon resonance (SPR) of TNF-α immobilized on a sensor chip, and by an ELISA technique able to detect TNF-α oligomers in solution. Validation studies, carried out with sensor chips bearing TNF-α oligomers or bovine albumin monomers, verified that: (a) TNF-α can be immobilized in the oligomeric form onto sensor chips; (b) the covalent linkage between TNF-α and sensor chips is stable under the experimental conditions: (c) TNF-α monomers are present on the sensor chips after dissociation; (d) immobilization and dissociation rate constant (kdiss) measurements are reproducible. The kdiss of recombinant TNF-α, measured under non denaturing conditions at pH 7.4 by BIA and ELISA were in good agreement, being 0.92×10−5/s and 1.1×10−5/s respectively (corresponding to a half life of about 20.9 h and 17.5 h, respectively). The dissociation rate was found to be significantly affected by the presence of detergents and by the pH of the solution, suggesting that TNF-α, at low concentrations, exists in solution with different molecular forms depending on the time of storage and buffer composition. Real-time BIA is rapid and does not require particular antibodies or reagents. Thus, the stability of the quaternary structure of natural or recombinant TNF-α from human or animal species as well as that of other oligomeric cytokines can probably be studied using this method." @default.
• W2068960211 created "2016-06-24" @default.
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• W2068960211 date "1993-11-01" @default.
• W2068960211 modified "2023-09-24" @default.
• W2068960211 title "Kinetic analysis of TNF-α oligomer-monomer transition by surface plasmon resonance and immunochemical methods" @default.
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• W2068960211 doi "https://doi.org/10.1016/s1043-4666(05)80002-x" @default.
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