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- W2068965514 abstract "One process identified as detrimental during liver preservation is proteolysis. The aim of this study was to test two preservation media, the University of Wisconsin (UW) solution and a simplified variant so-called Cochin (Co) solution with respect to their potential antiproteolytic effect. A model of isolated rat liver perfused after 24-h cold storage was used. Graft viability was assessed on biochemical and functional parameters. Proteolysis was assessed on amino acid metabolism. Survival of rats following orthotopic transplantation of 18-h cold-stored livers was determined. Proteolysis, assessed on valine release in the preservation solution, was limited in UW (0.12±0.01 versus 0.18±0.03 μmol/g liver, p<0.05). The protective effect of UW extended to reperfusion after storage and valine uptake was maintained in UW-stored livers but decreased in Co-stored organs (−12.39±5.93 versus −1.84±5.51 nmol/min/g liver). Enzyme release, portal flow rate and bile flow rate were similar in both groups. However, survival after orthotopic transplantation confirmed the superiority of UW over Co. These results demonstrated the antiproteolytic effect of UW solution in cold-stored rat livers. Amongst components omitted in the simplified UW solution, hydroxyethylstarch was identified as the more probable support of this effect." @default.
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- W2068965514 date "1998-08-01" @default.
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- W2068965514 title "Effect of two storage solutions on proteolysis in isolated rat livers" @default.
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- W2068965514 doi "https://doi.org/10.1016/s0378-5173(98)00151-3" @default.
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